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Attaullah et al

Greener Journal of  Agricultural Sciences Vol. 2 (5), pp. 212-219, September 2012

 ISSN: 2276-7770 © 2011 Greener Journals

Research Paper

Manuscript Number:08011229

 

Comparison of Different Techniques for the Isolation of Rhizoctonia solani from Infected Potato Tubers.

 

1Attaullah1, 2Shaukat Hussain1,3Farhad Ali1,3Amjad Khan and *2Gulzar Khan

 

1Khyber Pukhtunkhwa Agriculture University Peshawar

2North West Institute of Plateau Biology Chinese Academy of sciences, Xining 810001, China

3Gomal University KPK, Dera Ismail Khan

E-mail: attaullah.pathology@gmail.com

 

        *Corresponding Author’s email: gulzar_khan92 @ yahoo. com


Abstract:

The effect of surface disinfection, temperatures, nutrients and chemicals on sclerotial germination of Rhizoctonia solani from potato tubers were studied on potato dextrose agar (PDA). Germination occurred from tuber pieces bearing sclerotia pre-treated for one min with 0.1% of mercuric chloride. Tuber pieces pretreated with 0.5% and 1%of mercuric chloride for various lengths of times, including 15 sec, 30 sec and one min showed no sclerotial germination on PDA. Similarly sclerotial germination did not occur after pretreated with sodium hypochlorite for same concentrations and times. Sclerotial germination was optimal at a temperature of 250 C on PDA. Germination of sclerotia was best on PDA followed by cornmeal agar (CMA). Minimum mycelial growth occurred on water agar (WA) while sclerotial germination was minimal on malt extract agar (MEA) after one week of inoculation. Tuber pieces bearing sclerotia were also treated with 0.1 % of mercuric chloride, sodium hypochlorite, potassium hydroxide and ethanol for one min before inoculation but no sclerotial germination occurred on PDA except from pieces treated with mercuric chloride.
 
Key Words: Sclerotial Germination, Mycelial Growth, Inoculation, PDA.

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