EnglishFrenchGermanItalianPortugueseRussianSpanish

 

GREENER JOURNAL OF BIOLOGICAL SCIENCES

 

ISSN: 2276-7762             ICV: 5.99

 

 

Submitted: 10/06/2016                         Accepted: 15/06/2016                        Published: 06/08/2016

 

 

 

Research Article (DOI: http://doi.org/10.15580/GJBS.2016.4.061016103)

 

A Checklist of Phytoplanktonic Algae of Nyamuhinga Stream in Lake Kivu Basin (Eastern DR Congo)

 

*1Bisimwa Mubwebwe Arthur, 2Ombeni Shamba Espoir,

2Munundu Mangaza Aline, 2Irenge Bayubasire Emmanuel,

3Cishugi Balagizi Janvier and 2Cubaka Kabagale Alfred

 

1Limnology Laboratory, Department of Biology, Centre de Recherche en Sciences Naturelles de Lwiro (CRSN-Lwiro), D.S. Bukavu/DR Congo.

2Vegetable Physiology and Apply Microbiology Laboratory, Faculty of Sciences and Applied Sciences, Université Officielle de Bukavu (UOB), PO Box. 570 Bukavu, DR Congo.

3Biology Department, Section of Exact Science, Institut Supérieur Pédagogique de Kinyatsi (ISP-Kinyatsi), .PO Box.

 

*Corresponding Author’s Email: bismubwebwe@ gmail. com; Phone: (+243) 997 759 496 / (+243) 859 434 736

 

ABSTRACT

 

The phytoplankton diversity of Nyamuhinga stream was investigated at the first time from January to December 2014. It recorded a total of 135 species, belonging to four major divisions namely: Bacillariophyta (49.6%), Chlorophyta (21.5%), Cyanophyta (20.7%) and Pyrrophyta (8.2%). Comparatively, a higher number of species was recorded in the dry than in the wet season. Sixteen species of Bacillariophyta were reported during the all period of the sampling dominated quantitatively by Achnanthes lanceolata, Cyclotella kützingiana, Cyclotella meneghiniana, Cyclotella stelligera, Cymbella grossestriata, Diatoma elongatum, Diatoma vulgare, Fragilaria crotonensis, Gomphonema brunii, Gomphocymbella brunii, Navicula cocconeiformis, Nitzschia acicularis and Nitzschia pelagica. Seven species of Chlorophyta and five of Pyrrophyta were reported, but respectively dominated quantitatively by Ankistrodesmus falcatus, Cladophora rupestris, Cosmarium austral, Staurastrum dickiei, Euglena granulate and Peridinum inconspicuum. The paucity of phytoplankton composition in Nyamuhanga stream could be partly due to the poor light penetration into highly turbid water mostly in wet season.  

 

Keywords: Phytoplankton species, Community structure, Nyamuhinga stream, Season, Lake Kivu basin.

 

 

1. INTRODUCTION

 

Phytoplanktons are microscopic organisms that are suspended in water. They constitute the bedrock or basis of grazing food chains and food webs in surface water systems (UNEP, 1998; SPDC, 2001; Herring, 2005). Phytoplankton, as primary producers, forms the vital energy source at the first trophic tier. As they also serve as food to many aquatic animals, they also have an important role in the material circulation in aquatic ecosystems by controlling the growth, reproductive capacity and population characteristics of aquatic biota. Furthermore, their standing crops exhibit variations that depend on several factors, including: (i) the supply of major nutrients (mainly phosphorus and nitrogen); (ii) light availability; (iii) grazing by zooplankton; (iv) water mixing regimes; and (v) basin morphology (Reynolds et al., 2001; Gurung et al., 2006). 

Evaluation of phytoplankton community structure is essential, therefore, to evaluation of the water environment (Caljon, 1992; Lung’ayia et al., 2000; Onyema, 2008; Elijah et al., 2009; Bisimwa et al., 2013; Sarmento et al., 2006, 2007). Several studies on the pelagic algae of Lake Kivu were undertaken (e.g. Zanon, 1938; Damas, 1937; Kilham and Kilham, 1990; Haberyan and Hecky, 1987; Sarmento et al., 2006, 2007), but its tributaries remain without phycological information hence, such study remains important because majority of the riverine inhabitant most of the time depend on their water needs. For some published works on the aquatic algae of Lake Kivu effluents, we can quote those of Scaёtta (1928 in Sarmento et al. 2007), Bisimwa et al. (2009a, 2009b, 2013) and Bahati et al. (2015). This study aims to investigate the phytoplankton algae community of Nyamuhinga stream and hence document a checklist of encountered species for environmental biological monitoring. 

 

 

2. MATERIALS AND METHODS

 

2.1. Study area   

 

Nyamuhinga stream (17 Km long, of average depth 25 cm) is located, in South-West side of Lake Kivu (Eastern DR Congo), within latitudes 2°28’S and 2°27’S and longitudes 28°48’E and 28°50’E between 1469 and 1592 m asl (Irenge, 2013). Samples were collected from January to December 2014 on five stations selected from the upstream to the downstream to reflect the inputs of any other discharges to the stream that may influence water quality conditions (figure 1). Station 1 was a reference site located on upstream at Musigiko cemetery at about 1.3 Km of the spring between 2°28’46’’S-28°48’44’’E, 1728 m asl. Station 2 was close to Nyakavogo stadium between 2°28’40’’S-28°49’48’’E, 1607 m asl at 4.8 Km of the spring. Station 3 was situated at Quartier C, approximately at 9.6 Km from the spring between 2°28’00’’S-28°49’34’’E, 1542 m asl. It receives the sewage from Bagira. Station 4 was located at Nyaciduduma near the older sewage treatment of Bagira under the bridge which goes to Mbobero at 2°27’45’’S-28°49’51’’E, 1538 m asl. It is at about 13.2 Km from the spring. Station 5 was positioned on downstream at Kazingo near the Central Station of fuel tankers, approximately at 16.8 Km of the spring between 2°27’39’’S-28°50’11’’E, 1494 m asl.   

Nyamuhinga takes its source in Kabare territory at Kakoma-Mulwa, and it is the boundary between the Kabare and Bagira Township. In his way, Nyamuhinga collects all the wastewater and sewage from Bagira and flowing into Lake Kivu near the Central Station of fuel tankers. It is therefore a continuous and worrying risk to the environment and the health of surrounding populations. Its catchment, favorable to agriculture and livestock, is located in an area whose climate is wet type two seasons: a rainy season from September to May and a dry season from June to August. The average temperature and annual rainfall of the area are respectively 19oC and 1403 mm (Bisimwa et al., 2014).

 

2.2. Phytoplankton Sampling

 

Samples were collected between 9.00 am and 11.00 am every month from five different points on the stream. Physicochemical properties (temperature, transparency, pH and dissolved oxygen) were measured on each sampling day. Phytoplankton samples were collected with a round silk cloth net of mesh size about 55 μm. The diameter of the net is 40 cm held open by a metal frame and attached to a wooden handle with a 150 mL bottle attached to the distal end. The net was towed through the water for qualitative phytoplankton sampling, 30 litres of water was poured into net to concentrate the sample. Samples were then transferred from the bottle attached to the end of the sampling net to sampling bottles and immediately preserved with 4% formalin. Phytoplankton was identified using keys from Hustedt (1971), Whitford and Schumacher (1973), Patrick and Reimer, (1975), Prescott (1982), Germain, (1981), Ricard, (1987), Krammer and Bertalot (2000).

 

Figure 1. Location of sampling stations investigated in Nyamuhinga stream

 

 

2.3. Enumeration of Phytoplankton

 

The samples were concentrated to 10 mL by sedimentation processes, which enable phytoplankton to settle to the bottom of the transparent bottle. The bottle was agitated thoroughly before a drop (approximately 1 mL) sub sample was quickly taken with a wide-bore (3 mm of diameter) dropper. The sample was introduced carefully into a counting chamber and covered with a cover slid. Counts of various groups of organisms present were made with a compound microscope. Five sub samples were taken from each bottle and counted. The mean number of individuals per mL was computed from these sub samples. The number of organisms per litre was calculated from the following relationship:

 

 

Where: A = Number of organism/Litre of water; B = Organism/mL of concentrate;

            C = Volume of water filter; D = Volume of Concentrate

 

2.4. Community structure

 

To obtain the estimate of species diversity, three community structure indices were used. The species richness index (D) given by the equation (Margalef 1970):

 

 

Shannon-Weiner diversity index (H) using the following equation (Shannon and Weiner 1973):

 

 

Species equitability (J) with the following equation (Piélou 1975):

 

 

Where: pi = ni/N; ni = number of individuals of species i; S = total number of species;

             N = total number of individuals in a sample

 

 

3. RESULTS

 

3.1. Physicochemical characteristics

 

 

 

The mean values of physicochemical parameters of water in Nyamuhinga stream are presented in Table 1. The surface water temperature varied between 23.4oC-26.7oC during the study period. Temperature had minimum mean value (24.6oC) in the wet season while it peaked in the dry season, with a mean value of 25.1oC. Hydrogen ion concentration (pH) ranged between 6.2 and 7.46 throughout the sampling period; it is essentially  acid  as  pH mean value is 6.98. Electrical Conductivity had mean minimum value (630.0 μS/cm) in the dry season and the mean maximum (642.6 μS/cm) in the wet season. For the flow, mean value peaked and was least (0.71 m3/s) in wet season. The Nitrate range between 0.02-1.11 mg/L with the least mean value (0.04 mg/L) recorded in dry season, while the Ammonium mean value does not significantly change in dry and wet season. In general, Dissolve Oxygen content with mean value (5.8 mg/L) was recorded in the two seasons. The Biological Oxygen Demand value ranged from 0.2-4.7 mg/L while Chemical Oxygen Demand values ranged from 6.0-37.6 mg/L for all period study. The Total Suspend Solids mean values recorded throughout the study period was generally low with the mean highest value (0.32 mg/L) recorded in the wet season.

 

3.2. Phytoplankton community structure

 

Four major algal groups were represented in the micro-flora of sampled areas of the Nyamuhinga stream. These were the Bacillariophyceae, Chlorophyceae, Cyanophyceae and Pyrrophyceae. A total of 135 species from 45 genera were recorded. Diatoms were the most abundant group making up 67 species from 20 genera. The green algae recorded 29 species from 13 genera, cyanobacteria with 28 species from 7 genera and euglenoids represented by 11 species from 5 genera (figure 2). Table 2 shows a checklist of Nyamuhinga phytoplankton species and their classification. Shannon-Wiener information (H’) values were respectively 4.33 and 4.25 in dry and wet seasons while species equitability (J) value were 0.98 recorded in wet and 0.96 in dry season. Species richness (D) recorded its highest value (11.3) in dry season. Dominance of phytoplankton samples by a few species was reflected by low equitability (J) value recorded and since, Margalef’s (D) value is influenced by the number of species and individuals, high (D) values recorded in dry season reflected high species number and relatively low numbers of individuals. Variations between community structure analysis in Nyamuhinga stream are shown in figure 3. In Nyamuhinga stream, higher H values observed could be attributed to high J value recorded (table 2).

 

 

 

Bacillariophyceae

 

The occurrence of diatoms was more in the dry season than in the wet season. The bacillariophyceae were the predominant groups at all stations for the duration of the study in terms of phytoplankton species diversity. Eighteen diatom species were recorded with the pennate forms being more diverse than the centric forms. Cyclotella kützingiana, Cyclotella meneghiniana and Cyclotella stelligera were the more abundant and frequently occurring centric species recorded. More frequently occurring pennate diatoms included Achnanthes lanceolata, Cymbella grossestriata, Diatoma elongatum, Diatoma vulgare, Fragilaria crotonensis, Gomphocymbella brunii, Navicula cocconeiformis, Nitzschia acicularis and Nitzschia pelagica.

 

Chlorophyceae

 

A total of twenty-nine species were recorded for the green algae with fifteen in the dry season and twenty-one in wet season. Ankistrodesmus falcatus, Cladophora rupestris, Cosmarium austral and Staurastrum dickiei were the more abundant and frequently occurring species recorded during the study period.

 

Cyanophyceae

 

The cyanobacteria recorded twenty-eight with fifteen in the dry season and sixteen in wet season. No one was abundant, but three species namely Chroococcus sp., Merismopedia elegans and Oscillatoria geminate were frequently occurring during the study period.

 

Pyrrophyceae

 

The euglenoids were represented by eleven species, ten in the dry season and six in the wet season. Only Euglena granulates and Peridinum inconspicuum were the more abundant and frequently occurring species.

 

 

 

 

 

DISCUSSION AND CONCLUSION

 

In Nyamuhinga stream, phytoplankton diversity was higher in the dry (89 species) than the wet season (77 species) and diatoms were the more important group among the phytoplankton categories recorded. Egborge and Sagay (1979), Chindah and Pudo (1991) and Adesalu (2010) have reported that phytoplankton production in Nigeria’s river was high and principally dominated by diatoms. Similar dominance of diatoms among phytoplankton assemblages have been reported by other researchers in the pelagic zone of Lakes Kivu and Tanganyika (Damas, 1937; Kilham and Kilham, 1990; Haberyan and Hecky, 1987; Caljon, 1992 and Sarmanto et al., 2006, 2007). Similarly, Bisimwa et al. (2009b, 2013) and Bahati et al. (2015) reported diatoms dominating the epibenthic algae of Kahuzi-Biega National Park’s streams and the epilithic periphytons of Lwiro region’s rivers. 

The pennate diatoms (60 species) of Nyamuhinga stream were more in number than the centric diatoms (7 species) attributed to the numerous pennate forms recorded to the effect of tidal mixing that probably scours up the phytobenthic forms into the plankton of Lake Kivu affluents. The reduced phytoplankton diversity in the wet season may be linked to the low water clarity which reduces the amount of light available to the planktonic algal component for photosynthesis. Sarmanto et al. (2006, 2007) have also reported similar inferences for the pelagic algae of Lake Kivu, Bisimwa et al. (2013) and Bahati et al. (2015) reported the epibenthic algae of Kahuzi-Biega National Park’s streams and Bisimwa et al. (2009b) reported the epilithic periphytons of Lwiro region’s rivers. 

Whereas diatoms were prominent in both seasons, the green algae and the cyanobacteria were important in terms of diversity in the wet season. A good number of the species encountered for this study have been recorded before now in Lakes Kivu and Tanganyika region (Damas, 1937; Caljon, 1992; Sarmanto et al., 2006, 2007; Bisimwa et al., 2009b, 2013 and Bahati et al., 2015). Notable encountered genera for the study were Achnanthes (4 species), Cyclotella (4 species), Cymbella (4 species), Gomphonema (4 species), Closterium (4 species), Merismopedia (4 species), Navicula (9 species), Nitzschia (8 species), Pediastrum (5 species), Euglena (5 species), Microcystis (4 species), Lyngbya (4 species) and Oscillatoria (12 species). These species have been reported as prominent in other studies (Egborge and Sagay, 1979; Chindah and Pudo, 1991; Adesalu, 2010; Bisimwa et al., 2009b, 2013 and Bahati et al., 2015). 

In this study, the Euglenophyceae had a wider distribution and two of the organic pollution indicators species observed were Euglena acus and Phacus orbicularis (Adesalu, 2010). The observation of more diatoms than green algae and cyanobacteria in this study conformed to typical trend in tropical water bodies (Chindah and Pudo, 1991; Adesalu, 2010; Bisimwa et al., 2009b, 2013 and Bahati et al., 2015). Wetzel (1983) and Adesalu (2010) reported that chlorococcales inhabit water of differing salinity and alkalinity. The low desmids recorded could be a pointer that the stream is poor in its ionic composition (Nwankwo, 1996; Adesalu, 2010) because, high diversity of desmids is an indication that the water body is largely unpolluted (Egborge and Sagay, 1979; Adesalu, 2010) and this  is  supported with the presence of Euglena acus. According to Caljon (1987), Conforti (1991) and Adesalu (2010) this group is characteristic of eutrophic or nutrient rich water bodies and these abundance is a pointer that probably, Nyamuhinga stream is organically polluted, which could be due to the wastewater and the sewage discharged into and to intense anthropogenic activities in its catchment. So far, no work has been done on the algal flora of Nyamuhinga stream, hence all these forms constitute new records and this study has provided baseline data for the Nyamuhinga stream and more work needs to be done. 

 

 

ACKNOWLEDGEMENT

 

The authors are grateful to the Vegetable Physiology and Apply Microbiology Laboratory, Faculty of Sciences and Applied Sciences, Université Officielle de Bukavu (UOB) for logistics support and use of its facilities. 

 

 

REFERENCES

 

Adesalu, T.A., 2010. Phytoplankton Dynamics of River Oli in Kainji Lake National Park, Nigeria during dry season. International Journal of Botany 6(2): 112-116 

Bahati, W., Bisimwa, K., Baguma, M., Lutwamuzire, C., Ndakala, M. and Bisimwa, M.A., 2015. Epibenthic Algal Assemblages of Kahuzi-Biega National Park Streams (East of DR Congo). International Journal of Innovation and Scientific Research, Vol. 15, 432-442

Bisimwa, M.A., Baluku, B. & Ngera, M.F., 2009 a. Estimation de la qualité des eaux des rivières Lwiro et Kabindi (Est de la RD Congo) à l’aide d’indice diatomique. Cahier du CERUKI, Numéro Spécial CRSN-Lwiro, 62-73

Bisimwa, M.A., Ngera, M.F. & Baluku, B., 2009b. Inventaire préliminaire du périphyton épilithique des cours d’eau de la région de Lwiro, Sud-Kivu, RD Congo. Cahier du CERUKI, Numéro Spécial CRSN-Lwiro, 74-82

Bisimwa, M.A., Ngera, M.F., Bisimwa, K., Bagalwa, M., Mushayuma, N., 2013. A Preliminary Checklist of Epilithic Algae of Kahuzi-Biega National Park, Democratic Republic of the Congo. Greener Journal of Biological Science, Vol. 3 (8), 282-291

Bisimwa, M.A., Mwanuzi, F. and Nobert, J., 2014. Water Quality Management in Lake Kivu Basin, DR Congo: Estimation of Nutrient and Sediment Loading into Lake Kivu from Bukavu sub-basin. Lambert Academic Publishing, 128p

Caljon, A.G., 1992. Water quality in the Bay of Bujumbura (Lake Tanganyika) and its influence on phytoplankton composition. Min. Internat. Verein. Limnol. 23: 55-65

Caljon, A.G., 1987. A recently ladlocked brackish water lagoon of Lake Tanganyika: Physical and chemical characteristics and spatio-temporal distribution of phytoplankton. Hydrobiologia, 153: 55-70

Chindah, A.C. and Pudo, J.K., 1991. A preliminary checklist of algae found in plankton of Bonny River in Niger Delta, Nigeria. Fragmenta Floristica et Geobotanica, 36(1): 117-126

Conforti, V., 1991. Taxonomic study of Euglenophyta of highly polluted river of Argentina. Nova Hedwigia, 53: 73-98

Damas, H., 1937. Quelques caractères écologiques de trios lacs équatoriaux: Kivu, Edouard, Ndalaga. Annales de la société royale zoologique de Belgique 68: 121-135

Egborge, A.B.M. and Sagay, E.G., 1979. The distribution of phytoplankton and zooplankton in some Ibadan freshwater ecosystem. Pol. Arch. Hydrobiol., 26: 323-335

Elijah., O.O., Mucai, M., Shivoga, W.A., Scott, N.M., Rasowo, J. and Chege, C.N., 2009. Spatial and seasonal variations in phytoplankton community structure in alkaline-saline Lake Nakuru, Kenya. 14, 57-69

Germain, H., 1981. Flore des diatomées. Eaux douces et saumâtres. Boudée et Cie, Paris, 443p

Gurung, T.G., Dhakal, R.P. and Bista, J.D., 2006. Phytoplankton primary production, chlorophyll-a and nutrient concentration in water column of mountainous Lake Phewa, Nepal. Lake reserve. Manage. 11, 141-148

Haberyan, K.A. and Hecky, R.E., 1987. The late Pleistocene and Holocene stratigraphy and paleolimnology of Lakes Kivu and Tanganyika. Palaeogeography, palaeoclimatology, palaeoecology 61: 169-197

Herring, D. (2005). What are phytoplankton. Earth observatory NASA/GSFC Security and Private State. 2p

Hustedt, F., 1971. Kryptogamen-flora von Deutschland, A-sterreichs under Schweiz 1st Edn, A Kademiscehe Vergasell Schaft M.L.H., Leipzing, 816p

Irenge, B., 2013. Evaluation des qualités physico-chimiques et biologiques des eaux de la rivière Nyamuhinga (Bukavu/RD Congo). Mémoire de Maitrise en Sciences de l’Environnement. Université Evangélique en Afrique (UEA), Faculté des Sciences d’Agronomie, 70p

Kilham, S.S. and Kilham, P., 1990. Endless summer: internal loading processes dominate nutrient cycling in tropical lakes. Freshwater biology 23: 379-389

Krammer, K. and Bertalot, H., 2000. Bacillariophyceae. Berlin. 482p

Lung’ayika, H.B.O., M’harzi, A., Tackx, M., Gichuki, J. and Symoens, J.J., 2000. Phytoplankton community structure and environment in the Kenyan waters of Lake Victoria. Freshwater Biology, 43: 529-543

Margalef, R., 1970. Perspective in Ecological Theory. University of Chicago Press. Chicago. 111p

Nwankwo, D.I., 1996. Phytoplankton diversity and succession in Lagos Lagoon, Nigeria. Arch Hydrobiol., 135: 529-542

Onyema, I.C., 2008. A Checklist of Phytoplankton Species of the Iyagbe Lagoon, Lagos. Journal of Fisheries and Aquatic Science 3: 167-175

Patrick, R. and Reimer, C.W., 1975. Reimer, The diatoms of the United States. Monograph of the Academy of Natural Sciences of Philadelphia, 13 (2), 213p

Piélou, E.C., 1975. The measurement of diversity in different types of biological collections. J. Theoret.Biol., 10: 370-373

Prescott, G.W., 1982. Algae of the Western Great Lakes Area with an Illustrated Key to the Genera of Desmids and Freshwater Diatoms. 1st Edn, Brown Co. Publishers, Dubuque, Iowa, 977p

Reynolds, C.S., Irish, A.S. and Eliot, J.A., 2001. The ecological basis for stimulating phytoplankton responses to environmental change (PROTECH). Ecol. Model. 140, 271-291

Ricard, M., 1987. Atlas du phytoplancton marin. Vol.2. Diatomophycées. Ed. CNRS, Paris, 297p

Sarmento, H., Leitao, M., Stoyneva, M., Compere, P., Coute, A., Isumbisho, M. and Descy, J.P., 2007. Species diversity of pelagic algae in Lake Kivu (East Africa). Cryptogamie, Algol., 28(3): 245-269

Sarmento, 2006. Phytoplankton ecology of Lake Kivu (Eastern Africa). Journal of plankton research 28(9): 815-829

Shannon, C.E. and Weiner, W., 1973. The mathematical theory of communication. University of Illionois, Illinois, USA

SPDC, 2001. Environmental Impact Assessment Study (EIA) of seismic survey in the Igbomotoni prospect of OML33 prepared for subsurface developmental services (DTE-GPH). Suvricorn Services Nigeria Limited 81p

UNEP, 1998. Small Island Environmental Management, Unit DC (water pollution), Geneva.

Wetzel, R.G., 1983. Limnology. 2nd Edn., Saunders College Publishing, Philadelphia.

Whitford, L.A. and Schumacher, G.J., 1973. A Manual of Fresh Water Algae. Spark Press, Raleigh, N.C., 324p

Zanon, 1938. Diatomee della regione del Kivu (Congo Belga). Commentationes Pontifica academia scientiarium 2: 535-668

 

 

Cite this Article: Bisimwa MA, Ombeni SE, Munundu MA, Irenge BE, Cishugi BJ and Cubaka KA (2016). A Checklist of Phytoplanktonic Algae of Nyamuhinga Stream in Lake Kivu Basin (Eastern DR Congo). Greener Journal of Biological Sciences, 6(4):068-078, http://doi.org/10.15580/GJBS.2016.4.061016103