Greener Journal of Microbiology and Antimicrobials

Open Access

Chengalroyen and Dabbs

Greener Journal of Microbiology and Antimicrobials Vol. 2 (1), pp. 007-014, March 2014.

 ISSN: 2354-2284 © 2013 Greener Journals

Research Paper

Manuscript Number: 010814023


The use of pAL5000 Replicon Vectors to Transform Rhodococcus and GordoniaSpecies


M.D. Chengalroyen1* and E.R. Dabbs2


1Department of Genetics, University of Witwatersrand, Johannesburg, Postal address:

P.O. Box 1038, South Africa.

2Department of Genetics, University of Witwatersrand, Johannesburg, South Africa.


Email: 2Eric.Dabbs @ wits. ac. za


*Corresponding Author’s Email: mchengalroyen @ gmail. comTel: 0114899134


pAL5000 based vectors, conventionally used to transform Mycobacterium species were used in this study to transform Rhodococcus and Gordonispecies. Adequatetransformation efficiencies were obtained ranging between 4.5x103 – 2.8x106.Additionally, a pAL5000 based vector with suicide function was generated. This vectorwas transformed into RhodococcusMycobacterium and Gordonispecies and was maintained as a stable construct.

Keywords: pAL5000, RhodococcusGordonia, suicide vector.

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