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Greener Journal of Agricultural Sciences

ISSN: 2276-7770; ICV: 6.15

Vol. 6 (10), pp. 285-293, November 2016

Copyright ©2017, the copyright of this article is retained by the author(s)

http://gjournals.org/GJAS

 

 

 

 

Research Article (DOI: http://doi.org/10.15580/GJAS.2016.10.092316144)

 

Aflatoxin Contamination of Stored Groundnut Kernel in Sokoto State, Nigeria

 

 

*1Salau IA, 2Shehu K, 2Muhammad S and 3Umar RA

 

 

*1Department of Biology, Shehu Shagari College of Education, Sokoto Nigeria.

2Department of Biological Sciences, Usmanu Danfodiyo University, Sokoto Nigeria.

3Department of Biochemistry, Usmanu Danfodiyo University, Sokoto Nigeria.

 

 

 

 

ARTICLE INFO

ABSTRACT

 

Article No.: 092316144

DOI: 10.15580/GJAS.2016.10.092316144

 

Aflatoxins (AF) are a group of fungal toxins that contaminate foods and feeds with adverse health impact on human beings and animals. A total of sixty three (63) samples of Groundnut kernels from three (3) Agricultural zones of Sokoto State, were analysed for the presence of Aflatoxins B1 (AFB1), B2 (AFB2), G1 (AFG1) and G2 (AFG2) using High Performance Liquid Chromatography (HPLC). The Aflatoxin was detected in 82.5%, the total Aflatoxin concentration ranged between 0.9-646.0 μg/kg. All four types of AF were detected from all samples, out of the total samples analysed 38.10% contained AF levels above the Nigerian (20 μg/kg) limits for AF, while 66.7% samples had AF concentrations above the European Union maximum tolerance level of 2μg/kg for AFB1 and Total Aflatoxin(Tot AF) of 4μg/kg. The result shows that, there were significant (P > 0.05) differences between the samples analysed in the state, as regards to AFB1. The results also revealed that Groundnut kernel in the study area have high frequency of contamination (82.5%). However, awareness on danger of Aflatoxin contamination by all stakeholders is the key in effective management of the problem.Relevant quality control units must be reactivated to assess the quality of the Groundnut kernels from which other products are made.

 

 

Submitted: 23/09/2016

Accepted: 05/10/2016

Published: 10/11/2016

 

*Corresponding Author

Salau IA

E-mail: ibrahimasalau @gmail .com

Phone: +234(0)8053569551

 

Keywords:

Aflatoxin, Contamination, Store Groundnut kernel and Agricultural zones

 

 

 

 

 

 

INTRODUCTION

 

Mycotoxins are  secondary  metabolites  present in  agricultural  products  worldwide that are produced  by  filamentous  fungi on invasion of the host tissue and are capable of  causing mycotoxicoses on ingestion by human or animals (Glenn, 2007). Mycotoxins are small and quite stable molecules which are extremely difficult to remove or eradicate and maintain their toxic properties along the tropic level in a food chain (Domsch, 2007). Approximately 400 secondary metabolites with toxigenic potential are produced by over 100 moulds. Identified mycotoxin according to Glenn (2007) includes Aflatoxins, fumonisins, ochratoxin, trichothecenes, zearalenone, penitrems and ergot alkaloids.

Aflatoxins (AF) are one of many natural occurring mycotoxins that are found in soils, foods, humans, and animals. Derived from the fungus, the toxigenic strains of Aflatoxins are among the most harmful mycotoxins. Aflatoxins are found in the soil as well as in grains, nuts, dairy products, tea, spices and cocoa, as well as animal and fish feeds (Waliyar et al. 2005). There are six forms of Aflatoxin: Aflatoxin B1 (AFB1), Aflatoxin B2 (AFB2), Aflatoxin G1 (AFG1), and Aflatoxin G2 (AFG2) are found in plant-based food, while Aflatoxin M1 (AFM1) (metabolite of AFB1) and Aflatoxin M2 (AFM2) are found in foods of animal origin. B1 is the most harmful form due to its direct link to human liver cancer (Leslie et al., 2006). The Aflatoxin problem has been recognized as one of the biggest challenges to food and nutrition security, trade, and health across the African continent. Aflatoxins are highly carcinogenic toxins that are produced by strains of the fungi Aspergillus flavus and A. parasiticus. In most West African countries, Groundnut, an important crop used in various forms including as a basic food and a cash crop, and which is one of the most susceptible crops to Aflatoxin, has been the worst hit. Since 1960, Groundnut production and exports from the West African region have been declining mostly due to Aflatoxin contamination of Groundnuts and Groundnut products. Small holder farmers are most affected, because they are highly dependent on the Groundnut production as it generates 60% of rural cash incomes in a number of countries in the region (Akano and Atanda 1990).

Groundnut (Arachis hypogaea) is an economically important crop grown in many parts of the world. Nigeria produced a total of 2.96 million metric tons from approximately 2.3 million hectares in 2011 making it the third largest producer in the world after China and India, the first and second producers, respectively (FAOSTAT 2013). Groundnut is used as human food and animal feed. It is also used for oil production and for many other purposes, and these underscore its importance to the agricultural sector (Bankole et al. 2005).

           A major constraint to Groundnut production and trade is the susceptibility of the crop to fungal invasion especially by aflatoxigenic fungi which subsequently produce Aflatoxin in the kernels (Mutegi et al. 2009; Soleret al. 2010). The affected kernels thus lose their quality and market value due to Aflatoxin contaminations. As stated earlier, areas of concern for Groundnuts revolve primarily around contamination by Aflatoxin, a class of mycotoxins produced mainly by Aspergillus flavus and A. parasiticus, in addition to cross-contamination from sources that introduce pathogens to Groundnuts after processing (Chang et al. 2013) In Nigeria, Groundnut is traditionally consumed in its boiled, roasted or other processed form to improve its aroma, flavour and texture, and also destroys contaminating microorganisms.

Nigeria has experienced high recorded Aflatoxin exposure levels in humans and has also reported the highest estimated number of cases of hepatocellular carcinoma (HCC-liver cancer) attributable to Aflatoxin (Liu and Wu, 2010) in the whole world. In recent years and in Nigeria, there have been growing concerns over the health hazard on consumption of Agricultural products contaminated with mycotoxin (Aflatoxin in particular) with deleterious consequences to human health. Poor system of farming, processing and storage facilities are factors that lead to the contamination of such products by Aflatoxin producing fungi (WHO, 2013)

The death of some children who consumed mouldy Groundnut cake in Ibadan was suspected to be due to aflatoxicosis (Ikeorah and Okoye 2005).

Aflatoxin determination and identification remain a priority before establishment of any health implication and control system. There are no reports on the incidence of Aflatoxin in Groundnut in Sokoto State which is one of the major Groundnut producing states in the country. For Nigeria to improve its competitiveness in Groundnut trade, the nation must generate data on the occurrence of Aflatoxin in the crop so as to establish whether the levels are safe for human and animal consumption and to further adopt measures to control their contamination levels.

The aim of this research work is to evaluate the presence, incidence, frequency and levels of Aflatoxin contamination of Stored Groundnut kernel in Sokoto State, Nigeria.

 

 

MATERIALS AND METHODS

 

Study Area

 

The study was conducted in Sokoto State, which is located in the extreme North-western part of Nigeria. The State geographically lies along longitude 110 301 to 130 501 East and latitudes 4o to 61 North and covers a total land mass of 26,648.48 square kilometres. Sokoto State shares boundary with Kebbi State to the south, Zamfara State to the east and the Republic of Niger to the north. The State has an estimated population of about 4,742,459 people as of 2016 with 99.9 persons per square kilometre, and 3% growth rate annually based on 2006 population census (NPC, 2007). Occupation of city inhabitants includes farming, trading, commerce, with a reasonable proportion of the population working in private and public sectors (MOI, 2008). The soil is predominantly a ferruginous tropical type, texturally sandy and pH of the soil ranges between 6 and 7. Rainfall starts late in June and ends early, in September but, sometimes extend to October. The average annual rainfall is between 550 - 1300 mm with peak in the month of August and a relative humidity of less than 20%. The highest temperatures of 45°C during the hot season are experienced in the months of March and April. Harmattan, (a dry cold and dusty) condition is experienced between the months of November and February (Abdullahi et al., 2009). Modern Sokoto city is a major commercial centre in leather crafts and agricultural products (MOI, 2008). There are 23 local government councils (LGC) in the State with Sokoto as the capital. The state is divided into four (4) agricultural zones viz Sokoto, Gwadabawa, Isa and Tambuwal.

 

Sampling

 

The State has four agricultural zones namely: Gwadabawa (Sokoto West), Isa (Sokoto East), Sokoto (Sokoto) and Tambuwal (Sokoto South). Each zone has between 5 - 6 local governments’ areas (Junaidu, 2005). For the purpose of this study, three (3) zones were randomly selected by balloting; Isa, Sokoto and Tambuwal. Simple random sampling technique was used for sampling of Groundnut kernel in the zones.

 

Selection of stores

 

Stores from selected local government areas were identified through local government departments’ of agriculture and traditional authorities.

 

Determination of Representative Sample Size

 

Research work in this area of study is scanty, as such this research work will served as a basis for the estimation of the representative sample size and it will be calculated using the formula below:

 

                                 S = x­­2PQ

                                 L2

 

                      S = Number of stores

                      X = the x score for a given confidence interval

                      P = Estimated prevalence

                      Q = 1-p

                      L = allowable error of estimation

 

In this research the desired confidence interval is 95% with an allowable error of estimation 0.05 the estimated prevalence that will be considered as 47% (Ezekiel and Sombie, 2014).

 

Collection of samples

 

A total of sixty three (63) stored groundnut samples were collected. Twenty one (21) samples each were collected from each Agricultural Zone. The samples were collected in sterile sampling bags at different storage centres. The samples were store at 0oC and immediately transported to the laboratory for Aflatoxin evaluation.

 

Determination of Samples Moisture

 

The moisture content of the samples was determined by rapid moisture analyser instrument (Gaucher model 600) supplied from Witeg, Germany. Weighed samples (250 gm) inserted into the instrument and the percentage moisture content from the digital reader was recorded as the average moisture content of each samples.

 

Analysis of Aflatoxin

 

The samples were subjected to extraction of toxins, clean up and analyzed for B1, G1 B2 and G2 according to the method described by Ehrlich and Lee (1984) without modification. Methylene chloride and phosphoric acid were used for the simultaneous extraction of AFB1, AFB2, AFG1 and AFG2. A separate portion of the initial methylene chloride/phosphoric acid extract was subjected to a specific clean-up procedure for Aflatoxin.

 

Extraction of Aflatoxin

 

Approximately 50 g portion of pulverized Groundnut kernel sample was weighed into a 500 ml Erlenmeyer flask and 25 ml 1M of the phosphoric acid and 250 ml of methylene chloride were added. The flask was placed on a mechanical shaker for 30 minutes and the content filtered under pressure on Buchner funnel fitted through an 18 cm circle rapid filter paper. Two hundred milliliter of the filtrate was collected and 50 ml aliquot was taken from the filtrate and placed in separate 100 ml Erlenmeyer flasks with glass stoppers, for Aflatoxin assay.

 

Aflatoxin Clean-up

 

The fraction for AFs analysis was subjected to a specific column chromatographic clean-up method. A column was set up with a glass wool and 150 ml of Dichloromethane (DCM) was poured into the column and emptied half way. Anhydrous sodium sulphate (Na2SO4) was added and the sides of the column were washed with DCM. Silica gel was added to the green line of column together with 80 ml of DCM and this was allowed to settle half way. Three scoops of anhydrous sodium sulphate (Na2SO4) were added and the DCM drained off to the top of the packed section of the column. About 50 ml of the filtrate was added and drained off to the top of the packed part of the column. The filtrate was defatted with 130 ml of N-hexane and 130 ml of ether sequentially, and each fraction drained out completely. Aflatoxins were extracted into 130 ml of ether/methanol/water (96:3:1, v/v/v) that was collected off column in a new beaker. The extract was evaporated to near dryness, put into sealed amber glass vials and stored at 0oC for a week until further analysis.

 

High Pressure Liquid Chromatography (HPLC)

 

Aflatoxins were analyzed on a Cecil 1100 series HPLC system equipped with a UV detector set at a wavelength of 365 nm as described by Cora et al. (2005). The Altraspher ODS column, 4.6 mm x 250 mm was used at ambient temperature of 25oC. Acetonitrile/ water/acetic acid (10:50:40, v/v/v) were used as the mobile phase pumped at a flow rate of 0.8 mL/min. The injection volume of both samples and standards used was 20 μL.

 

Aflatoxin Standard

 

The analysis was carried out with Aflatoxins standards (Sigma Chemical Company, St. Louis, MO, USA) of known concentrations. AFB1, AFG1 and AFB2, AFG2 eluted at distinct retention times of 1.673 min and 1.524 min respectively. Calibration curves with correlation coefficient (R2) of 0.91 and 0.99 were established for AFB1, AFG1 and AFB2, AFG2using a series of dilutions containing (0.004, 0.008, 0.012 and 0.016μg/ml) and (0.01, 0.02, 0.03 and 0.04 μg/ml) respectively for each standard. The limits of detection (LOD) were estimated as follows: known concentrations of Aflatoxin standards were prepared, successively diluted and subjected to HPLC until the minimum concentration at which the analyte could be detected was established. The LOD of the HPLC instrument with regards to both toxins was determined to be 0.21 and 0.18 μg/kg while the limits of quantification (LOQ) were estimated based on the standard deviations of response and slope; this gave 0.42 and 0.33 μg/kg respectively.

Quantification of the actual Aflatoxin in μg/kg is based on the following formula:

 

                                 Mycotoxin content (μg/kg) = S x Y x V

                                                                   W x Z

 

Where:

S = volume of standard with same colour intensity as sample (μl);

Y = concentration of mycotoxin standard used in μg/ml;

V = volume of solvent required to dilute sample contained in final extract;

W = effective weight (g) of original sample contained in final extract;

Z = volume of spotted sample equivalent to standard (μL).

 

Data analysis

 

The SPSS 21.0 (Windows version, IL, USA) was used for data analysis. Means for the distribution of concentrations of Aflatoxin were calculated and tested for significance at 95% confidence level by one-way ANOVA. The Duncan’s multiple range tests was further used to separate the means.

 

 

RESULT

 

Moisture content of Stored Groundnut kernel

 

The percentage moisture contents of Groundnut Kernel in relation to samples collected from different zones is shown in Fig 1. The results showed that the samples collected from Tambuwal zone had the highest moisture contents (13.5%), followed by Sokoto (10%) while samples from Isa zone had the lowest moisture content (8%).

 

 

 

Aflatoxin contamination of Groundnut kernels

 

The levels of Aflatoxin in Groundnut kernel obtained in Sokoto are presented in Table 1.  Sixteen samples out of twenty one (76.2%) in Sokoto zone were contaminated with the Aflatoxin. The highest levels of Aflatoxin were AFB1 (1.0 – 221.0 μg/kg), followed by AFG1 and AFB2 (1.5 – 113.0 and 1.1 – 76.0 μg/kg) respectively, the lowest concentrations of Aflatoxin were recorded for AFG2 (1.8 - 22.0 μg/kg). In the Isa zone of  Sokoto  state ,  eighteen samples out of twenty one (85.7%) were found to be contaminated with the Aflatoxin. The results indicated that the highest levels of Aflatoxin were AFB1 (1.3 – 156.0 μg/kg), followed by AFB2 and AFG1 (0.2 – 88.0 and 0.5 – 61.0 μg/kg) respectively, the lowest Aflatoxin concentrations were recorded for AFG2 (0.5 - 12.0 μg/kg). In Tambuwal zone, eighteen sample out of twenty one (85.7%) were contaminated with the Aflatoxin. The results reveal that the highest levels of Aflatoxin were AFB1 (0.9 – 450.5 μg/kg). This levels represent the highest range of concentration found in all the remaining sampling zones in the state, while the moderate levels were found on AFB2 and AFG1 (0.1 – 101.0 and 1.0 – 96.8 μg/kg) respectively, the lowest Aflatoxin concentrations were recorded for AFG2 (1.1 - 28.0 μg/kg).

All the contaminated Groundnut kernel samples were found to contain AFB1 at concentrations ranging from 0.9 and 450.0μg/kg. The least and highest concentrations of AFB1 were detected in samples obtained from Tambuwal (Table 2). However, the mean AFB1 concentration in samples analysed by locations were significantly (p > 0.05) different from each other. Out of 63 samples, more than thirty eight (38.1%) were contaminated with >20 μg/kg AFB1 [maximum acceptable limit (MAL) recommended by the National Agency for Food and Drug Administration and Control (NAFDAC). AFB1 and total Aflatoxin (ΣAFB1, AFB2, AFG1 and AFG2) in the Groundnut samples from three Agricultural zones of Sokoto States Nigerian were presented in Table 4. Aflatoxin B1 and total Aflatoxin levels were significantly (P < 0.05) different (means = 47.91and 84.28 μg/kg, respectively) and Groundnut from Tambuwal (means = 55.41 and 92.87μg/kg, respectively) and those collected from Isa (means = 39.96 and 64.00 μg/kg, respectively) and Sokoto (means = 48.36 and 95.96 μg/kg, respectively). Overall, 52 (82.5%) of the 63 samples were contaminated with Aflatoxins:  AFB1 (range = 0.9–450.0 μg/kg; mean = 47.91μg/kg) and total Aflatoxin (range = 0.9–646.0 μg/kg; mean = 84.28 μg/kg). The AFB1 maximum limit of 20 ng/g in Nigerian foods adopted by the National Agency for Food and Drug Administration and Control (FAO 2004) was exceeded in 24 (38.10%) of the Groundnut samples, while 42 (66.7%) of the samples had AFB1 and total Aflatoxin levels above the stipulated EU limit of 2 and 4 μg/kg, respectively (FAO 2004) (Table 2).

 

 

 

 

 

 

DISCUSSION

 

Moisture Content of Groundnut kernel

 

The moisture content observed in Groundnut kernel from this study indicates that 8% moisture was encountered in all the zones studied. This was conductive to encourage the growth of fungi in Groundnut (Woodroof 1984). Recent report of Halima (2000) also supports the view that fungi can grow on variety of situations and thrives under conditions of moisture, warmth and good supply of organic food which subsequently produce Aflatoxin. Contamination in stored groundnut is dependent on the moisture content of the harvested groundnut prior to storage. Therefore groundnuts which are stored before sale or use should be kept dry with a maximum moisture content of not more than 7 % and 13% for EU and SON respectively. The moisture content obtained from groundnut cake was found to be within the range as reported by Atanda (1990).

 

Aflatoxin contamination of Groundnut kernels

 

This study analysed 63 samples for the presence of Aflatoxins in stored Groundnut kernels and was determine to be 82% contamination with Aflatoxins in a concentrations range between 0.90μg/kg to 646.00μg/kg. Four types of Aflatoxin (AFB1, AFB2 AFG1 and AFG2) were detected. Of the Aflatoxins detected, AFB1 had the higher frequency (82.5%) of occurrence. Similarly, Patrick et al. (2009) found AFB1 and AFG1 as the most frequently occurring types of Aflatoxin in stored Groundnut samples. The Aflatoxin levels found in the Groundnut samples were much lower in value than those earlier reported in Groundnut by Afolabi et al., 2014, Kayode et al., 2013 and roasted Groundnut by Ogunsanwo et al. (2004) and Bankole et al. (2005). It’s also similar to the levels found in study carried out by Akano and Atanda 1990 and Ezekiel et al., 2013 in Lagos, Nigeria. And much higher than that by Odoemelam and Osu 2009, who investigated Aflatoxin B1 contamination of some food grains marketed in Nigeria. The result of this study is against the view of Afolabi et al., 2014, who considered Groundnut contamination in south western part of Nigeria may have been due to the soil types, time lag, post harvest handling and storage conditions during transportation of kernels from Groundnut producing regions (Northern Nigeria) to the South-Western zones where it is largely consumed. It is well known that the environmental factors (temperature, relative humidity and amount of rainfall) in south west is per greater than that northern Nigeria, and the can influence the production of Aflatoxin during storage, transportation and processing.

           In two different studies conducted in Nigeria, McDonald (1964) reported that Groundnuts cultivated in the northern parts of Nigeria were contaminated with Aflatoxin levels up to 2000μg/kg, while Akano and Atanda 1990 reported Aflatoxin B1 levels of between 37 – 455μg kg in Groundnut purchased from markets in Ibadan, Oyo State Nigeria. Adebajo and Idowu (1994) reported that most of the corn-Groundnut snack (‘donkwa’) contained Aflatoxins above 30μg/kg immediately after preparation. In some part of the world Park and Njapau 1989 reported AFB1 levels of between 20 – 200μg/kg in Groundnut from Senegal and Argentina, while Singh et al., (1982) reported AFB1 levels between 33 – 440μg/kg in Groundnut from India and Krishna et al., (2002) reports a levels of between 110-608μg/kg, in India Yameogo and Kassamba (1999) reported that seeds of Groundnuts from Burkina Faso inoculated with A.flavus excreted all the four major Aflatoxins, which peaked at 170 μg/kg after 6 days. Aflatoxin formation in Groundnut is favoured by prolonged end of season drought and associated elevated temperature (Rachaputi et al., 2002).

           Although the levels of AFB1 (0.9-450.0μg/kg) now being reported for Groundnut is within the range reported elsewhere, differences between these results and that obtained from other researchers may be due to variation in geographical origin, seasonal variation of the samples tested and weather conditions. It seems that difference in harvest and storage conditions as well as the agricultural practices (in each location) also influenced the level of Aflatoxin contamination of the Groundnut. Three environmental factors (temperature, relative humidity and amount of rainfall) influence the production of Aflatoxin in the field and during storage. Studies done on the effect of environmental conditions on Aflatoxin contamination of corn showed that, when the conditions were favourable, the occurrence of Aflatoxin was highly related to these factors (Farombi, 2003). Temperature is an important factor for growth of Aspergillusflavus. In a study on the effect of temperature on Aflatoxin production, Viquez et al., 2006 observed that Aflatoxin level was significantly affected by temperature. The study area has optimal temperature, humidity and light for the growth of Aflatoxigenic moulds. This may be responsible for the detection of Aflatoxin B1 in all the positive samples.

 

 

CONCLUSION

 

It is concluded that, Aflatoxin contaminated stored groundnut kernel in three Agricultural zones of Sokoto state, contamination in stored Groundnut kernel are dependent on the moisture content of the Groundnut kernel prior to storage. The result of this study revealed that Aflatoxin contamination is more in Isa and Tambuwal stored Groundnut kernel (85.7% each) than Sokoto (76.2%). More than 30% of Groundnut kernels are at levels greater than MAL of NAFDAC. Therefore Groundnuts which are stored before sale or use should be kept dry with a maximum moisture content of not more than 7 %, to prevent contamination by moulds or other microbes from the environment and consequent toxin liberation.

           The study recommended that Research has to be done to breed Groundnut varieties that are resistant to Aspergillus spp. which subsequently lead to Aflatoxin contamination of kernels and relevant quality control units must be reactivated to assess the quality of the Groundnut kernels from which other products are made.

 

 

ACKNOWLEDGEMENT

 

The authors are thankful to the laboratory staff of Crop protection Department, Institute of Agricultural Research ABU Zaria and Centre for Biotechnology, FUT Minna for their assistance for the conducts of this research study.

 

 

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Cite this Article: Salau IA, Shehu K, Muhammad S and Umar RA (2016). Aflatoxin Contamination of Stored Groundnut Kernel in Sokoto State, Nigeria. Greener Journal of Agricultural Sciences, 6(10): 285-293, http://doi.org/10.15580/GJAS.2016.10.092316144.