Greener Journal of Agricultural Sciences

ISSN: 2276-7770; ICV: 6.15

Vol. 6 (9), pp. 262-271, October 2016

Copyright ©2017, the copyright of this article is retained by the author(s)

http://gjournals.org/GJAS

 

 

 

 

Research Article (DOI: http://doi.org/10.15580/GJAS.2016.9.101416162)

 

Host Status of Plantain Hybrids FHIA 21 and PITA 3 for Populations of Radopholus similis and Pratylenchus coffeae in Côte D’ivoire

 

 

*1Vawa Otro Serge Theodore, 2Adiko Amoncho,

3Gnonhouri Goly Philippe, 4Otchoumou Atcho

 

 

1,402 BP 801 Abidjan 02, Université Nangui Abrogoua, Côte d’Ivoire.

2,301 BP 1536 Abidjan 01, Centre National de Recherche Agronomique, Côte d’Ivoire, Station de Bimbresso.

 

 

 

 

ARTICLE INFO

ABSTRACT

 

Article No.: 101416162

DOI: 10.15580/GJAS.2016.9.101416162

 

Background: The work herein reported has been conducted in the framework of the implementation of the strategy of food security strategy in Côte d’Ivoire. In this perspective, FHIA 21 and PITA 3, two high yielding plantain hybrids, resistant to black Sigatoka have been assessed relatively to their response to Radopholus similis and Pratylenchus coffeae.

Methods: FHIA 21 and PITA 3 were inoculated with increasing inoculum levels of the parasites under monoxenic conditions: 5, 10, 15 and 20 R. similis or P. coffeae/g roots and dixenic conditions: 3 R. similis and 3 P. coffeae; 5 R. similis and 5 P. coffeae; 10 R. similis and 10 P. coffeae; 10 R. similis and 5 P. coffeae; 5 R. similis and 10 P. coffeae/g roots. The cultivar HORNE 1 served as susceptible reference in both trials that were conducted in the glasshouse for eight weeks.

Results: FHIA 21 appeared resistant to R. similis (R=0.98) and susceptible to P. coffeae (R=2.99). As to PITA 3, it was susceptible to both R. similis (R=2.47) and P. coffeae (R=2.94). Host suitability was further confirmed when comparing nematode reproduction under the hybrids to that under HORNE 1. FHIA 21 was designated slightly resistant to R. similis with a reproduction of 40.22 % and susceptible to P. coffeae (83.27%). The susceptibility of PITA 3 was also confirmed to R. similis (83.27%) and P. coffeae (73.63%).

Conclusion: With R. similis, the threshold of 10 nematodes/g of roots commonly used for rational nematicide applications in banana cultivation was confirmed. In the light of the predominance of P. coffeae in banana and plantain agrosystems in Côte d’Ivoire, the new reference threshold  of 5 P. coffeae/g roots is recommended for an effective management of plant-parasitic nematode pressure.

 

 

Submitted: 14/10/2016

Accepted:  21/10/2016

Published: 31/10/2016

 

*Corresponding Author

Vawa Otro Serge Theodore

E-mail: vost_83@ yahoo. fr

 

 

Keywords:

Radopholus similis, Pratylenchus coffeae, Plantain hybrids, PITA 3, FHIA 21, HORNE 1, resistance, susceptibility, threshold

 

 

 

 

 

INTRODUCTION

 

Plantains are an important staple food and a source of income for more than 400 million people in nearly 120 countries in tropical and intertropical zones (Jones, 2000). In Côte d’Ivoire, the production of plantains is estimated at 1.6 million tons, making it the third food crop in volume after yam and cassava (Anonymous, 2016). Despite its importance, plantain production does not cover domestic demands with an average per capita consumption of 120 kg; the situation is further exacerbated by a sub-regional market of plantain steadily rising. The biotic and abiotic factors accounting for the low productivity of the crop have been well described (Lassoudière, 2009; Baker et al., 1997). Plant-parasitic nematodes represent one of the major biotic constraints on production (Bridge and al., 1995; Gowen and Quenehervé, 1990). Radopholus similis and Pratylenchus coffeae considered as the most destructive species (Sarah and al., 1996) can cause up to 60 % yield loss. For very long, the nematofauna associated with banana and plantain cultivations in Côte d’Ivoire has been dominated by R. similis; P. coffeae was barely detectable (Adiko, 1989; Sarah, 1989). However, the pest situation has greatly evolved, as the orchards of banana and plantain harbor the two most damaging parasitic nematode species of the crops (Adiko and N'guessan, 2001; Sarah, 2000; Gnonhouri and Adiko, 2008; Gnonhouri and al., 2009). The cohabitation of the two species represents a real threat to the economy of  banana agriculture, in general, and that of plantain, in particular. If chemical control which is generally recommended is a common practice in the commercial banana orchard, it is very rare in plantain cultivation that is dominated by small family farms. Moreover, land pressure and related problems do no longer allow the practice of extensive agriculture as an alternative measure for the management of  parasites  and  other  limiting  factors  ( Gowen  and  Quénéhervé 1990). According to the work of De waele (1996) and Pinochet (1996), Musa genepool offers credible opportunities that could be exploited for resistance breeding for nematode management. In this perspective and in the context of improving the productivity of plantain in Côte d'Ivoire, the Centre National de Recherche Agronomique (CNRA, Côte d’Ivoire) has selected and is in the process of disseminating two tetraploid varieties of plantain FHIA 21 and PITA 3, for both their high yield, their resistance to black Sigatoka (Anonymous, 1987; Vuylsteke and al., 1993). The study reported herein aims at evaluating the hybrid host status for the populations of R. similis and P. coffeae in Côte d’Ivoire.

 

 

MATERIALS AND METHODS

 

Area of study. The study was carried in the greenhouse, at the Research Station of the Centre National de Recherche Agronomique (CNRA), at Bimbresso, Côte d’Ivoire, West Africa. Bimbresso is located at 25 km in the West of Abidjan, at 5°19’14 N, 4°40’20 W and 23 m above sea level. Average annual temperature was 26 ± 1.34 °C.

 

Planting materiel: Three cultivars were  involved in the study. HORNE 1 is a triploid plantain, Musa spp. AAB group and is the cultivar commonly grown in Côte d’Ivoire. HORNE 1 is considered susceptible to plant-parasitic nematodes (Thiémélé, 2015. Personal communication). FHIA 21 and PITA 3 are plantain-like hybrids belonging to the genome group AAAB with a ploidy level of 4x. These hybrids result from crosses between AAB Plantain cv. AVP-67 x SH-3142 and AAB Plantain cv. Obino l’Ewaï x Calcutta 4, respectively. In-vivo micropropagated plantain plantlets of the three genotypes were used (Thiémélé and al., 2015). Forty five days old in-vivo plantlets at the end of the weaning period were transplanted in 6-liter buckets filled with sterile mixture of 5 parts of soil, 1 part of sand and 2 parts of peat.

 

Preparation of Nematodes inoculum: R. similis and P. coffeae were collected from roots of naturally infested plantain trees established at the experimentation site of the CNRA at Azaguié. Nematodes were extracted according to Seinhorst method (1950) and each species reared on sterilized carrot discs as described by (Boisseau and Sarah, 2008). After incubation at 28°C in darkness during 8 weeks, nematodes were extracted from carrot discs as follows. Carrot discs containing the reared nematodes were macerated in a blender for 10 seconds (two periods of 10 seconds separated by a 5 second interval). The resulting mixture was passed through two sieves of 106 and 25 µm respectively. Carrot tissue and debris collected on the 106 µm sieve were discarded, and nematodes on the 25 µm sieve were recovered in test tubes (Boisseau and Sarah, 2008). Three 1 ml samples of the resulting suspensions were pipetted in graduated counting dishes and number of each nematode species determined under a dissecting microscope. The total population density of each species was estimated by converting the mean value of samples into total volume.

 

Inoculation of plantain plantlets

 

In 1972, Guerout has determined a level of 10 R. similis per g of roots as the threshold for the management of nematodes in banana orchard. On that basis, the following treatments were applied on each plantain variety in the study herein reported.

 

In monoxenic inoculation

 

i)          Check (without nematodes);

ii)         5 nematodes/g roots;

iii)        10 nematodes/g roots;

iv)        15 nematodes/g roots;

v)         20 nematodes/g roots.

 

In dixenic inoculation

 

i)    Check (without nematodes);

ii)   6 nematodes /g roots  (3 R. similis and 3 P. coffeae);

iii)  10 nematodes /g roots (5 R. similis and 5 P. coffeae);

 

iv)  15 nematodes / g roots (10 R. similis and 5 P. coffeae);

 

v)   15 nematodes /g roots (5 R. similis and 10 P. coffeae);

 

vi)  20 nematodes /g roots (10 R. similis and 10 P. coffeae).

 

In order to calibrate the nematode suspension to be pipetted  for inoculation according to the  initial densities set above, 10 plantlets of each genotype were uprooted. Roots were delicately washed with tap water and the average fresh weight determined. Plantlets were inoculated one week after transplanting. For the inoculation per set, nematodes were collected from the calibrated suspension and applied with a micropipette in three holes (5 cm deep and 0.5 cm diameter) made in the soil around the pseudostem. Holes were reclosed with the same soil substrate and plantlets lightly watered. Each treatment was replicated six times. Treatment replicates were arranged in a completely randomized design in the greenhouse where temperature ranged from 27-28°C and humidity from 80-90 %.

 

Extraction and assessment of final nematode populations: Eight weeks after inoculation, trials were terminated. Plants were removed from the buckets, their roots washed with tap water, chopped and thoroughly mixed. A sample was collected for nematode extraction using the centrifugal-flotation technique described by Sarah and Boisseau (2008). Two ml aliquot of the suspension was pipetted in graduated counting dishes and number of nematode determined under a dissecting microscope. The total population density was estimated by converting the value of the aliquot into total volume.

 

Host suitability of FHIA 21 and PITA 3 to  R. similis et P. coffeae

 

The host suitability of the two hybrids  was assessed according to two criteria, the reproduction factor as defined by Oostenbrink (1966) and the host resistance classification system developed by Taylor (1967). According to Oostenbrink’s formula, nematode reproduction factor (R) is the ratio between the final and initial population (R = Pf/Pi). As to Taylor’s (1967) classification of host suitability, it compares the reproduction of the assessed genotypes against that of a known susceptible variety of the same species. In the present study, the reproductive capability of FHIA 21 and PITA 3 was compared to that of the reference cultivar HORNE 1. Nematode reproduction rate (T) under the genotypes compared to that under the reference cultivar was calculated as follows: T genotype x 100/T cultivar; T = (Pf-Pi)/Pi, with T genotype being the reproduction rate of the hybrid, Tcultivar the control reproduction rate, Pf the final population and Pi the original inoculum.

 

Assessment of the incidence of R. similis and P. coffeae on the vegetative growth of plantain genotypes

 

Eight (8) weeks after inoculation, the following parameters were recorded: plant height (cm), fresh root weight (g), leaf area (cm2) of the second leave. Leaf area was calculated according to Simmond’s formula (1962) (SF = L x l x 0.8), where L is the length and l the width of the leaf. The number of leaves produced per plant was also counted and the biomass (%) determined.

 

Data statistical analysis: Data consisting of nematodes and agronomic parameters were respectively subjected to log(x +1) and angular transformations prior to ANOVA analysis with statistica software 9.0 package. Means were compared using Fisher’s multiple range test (P ≤ 0,05).

 

 

RESULTS AND DISCUSSION

 

Host suitability of FHIA 21 and PITA 3 to R. similis and P. coffeae 

 

The reproduction factor of R. similis is significantly (P<0.05) lower on hybrid FHIA 21 (0.98) than PITA 3 (2.48) and the control cultivar HORNE 1 (2.98) (Table 1); the latter two being statistically equivalent. With P. coffeae, the reproduction factor was higher than to 2 and was comparable in all three genotypes. According to the classification of Oostenbrink (1966), hybrid FHIA 21 with a reproduction factor below one can be considered a poor host of R. similis. As to PITA 3 and HORNE 1, they appeared as good hosts of the population of the banana nematode in Côte d’Ivoire. The results herein challenge the report of the Honduran Fundation for Agricultural Research that characterized the hybrid as susceptible to both R. similis and P. coffeae (Anonymous, 1987). However, these results are in accordance with Lassoudière’s finding (2009) who has reported resistance of FHIA 21 to R. similis in commercial plantations of banana Cavendish in the Republic of Guinea. Resistance of FHIA 21 has also been documented in Cuba after 25 years of FHIA 21 cultivation by Almeres cited by Pocasangré (personal communication, 2015) . On the basis of the reproduction rate of P. coffeae on the hybrids, PITA 3 (R = 2.94) and FHIA 21 (R = 2.99) can be considered good hosts of the nematode species (P <0.05) (Table 1).

            On the basis of  host resistance classification system developed by Taylor (1967), FHIA 21 with a multiplication rate of R. similis of 40% of that under HORNE 1 was designated “slightly resistant” host to the banana nematode. PITA 3 has supported a multiplication rate of R. similis representing 80% of  that  on   HORNE 1; the former was classified as susceptible as the latter (Table 2). As to P. coffeae, the rates of multiplication were 73.6 % and 75 % were respectively recorded on PITA 3 and FHIA 21 (Table 3). Like the control cultivar HORNE 1, both hybrids can also be classified susceptible to P. coffeae.  From the results reported in the present study, and in the light of the recent evolution of the nematofauna associated with banana and plantain cultivation, , the parasitic pressure due to plant-parasitic nematodes in Côte d’Ivoire could be considered exacerbated,. Indeed, for very long the fauna of parasitic nematodes  on the crops, was dominated by R. similis. Recently however, nematode infection has evolved and is now characterized by the prevalence of P. coffeae in plantain and banana orchards (Adiko and N’guessan, 2001; Gnonhouri and Adiko, 2008; Gnonhouri and al., 2009). In the prevailing context, the expected impact from the dissemination of improved plantain varieties with a high production potential could be compromised. Therefore, efficient management measures of R. similis and P. coffeae must be envisaged. These measures require, among others, the determination of a new damage threshold of the parasites, notably that of the most dominant and aggressive nematode species, P. coffeae.

 

 

 

For the statistical analyses, reproduction factor data were arc sin(x/100) transformed. Averages in the same column followed by the same letter are not significantly different (P≤0.05) according to Fisher multiple range test.

 

 

 

For the statistical analyses, reproduction rate data were arc sin(x/100) transformed. Averages in the same column followed by the same letter are not significantly different (P≤0.05) according to Fisher multiple range test.

 

 

 

For the statistical analyses, reproduction rate data were arc sin(x/100) transformed. Averages in the same column followed by the same letter are not significantly different (P≤0.05) according to Fisher multiple range test.

 

 

Incidence of R. similis and P. coffeae on vegetative parameters of plantain hybrids FHIA 21 and PITA 3

 

Increasing inoculum levels of nematodes (0, 5, 10, 15 and 20 nematodes/g roots) was negatively correlated to the measured agronomic parameters (fresh weight of roots, stem growth, leaf area, biomass and leaf number). With R. similis, the incidence of nematode infection was generally recorded under the initial density of 10 nematodes/g fresh roots on FHIA 21 and PITA 3 (Table 4). As for P. coffeae, significant incidence of the nematode on all three plantain genotypes was noted from the initial density of 5 nematodes/g roots (Table 5). The result reported here with the initial density of 10 R. similis per gram of roots confirms the damage threshold set by Guérout (1972). That threshold has served as reference for the implementation of the warning strategy used in the framework of rational nematicide applications in commercial banana orchards in Côte d’Ivoire; strategy based upon the evolution of the nematode infestation in the field, especially that of the most damaging and predominante species Musa spp. in the country, R. similis (Sarah, 1989; Adiko and N' guessan , 2001). With P. coffeae on the contrary, the damage threshold was noted at 5 nematodes/g roots. All the vegetative parameters measured were significant at that inoculum level on the three plantain genotypes (Table 5). This variation in the incidence of two migratory endoparasitic nematodes may be due, in part, to the differential aggressiveness between the two species. Indeed, the greater damage capacity of P. coffeae can be related to the parasite ability to colonize all cellular compartments of the root system (Araya and De Waele, 2004). Furthermore, because of the amphimictic and parthenogenetic reproduction of P. coffeae, both male and female nematodes in the population attack the plant in addition to four juvenile stages (J1 to J4) that are also infectious (Pinochet and al., 1995). Moreover, the biological life cycle of P. coffeae (21 days) compared with that of R. similis (28 days) is shorter and the former species, more prolific. As for R. similis that has a parthenogenetic reproduction, males are rare, and only female and three juvenile stages (J1 to J3) are infectious (Stoffelen and al., 1999). All these factors could account for the damage threshold of 5 nematodes/g roots against 10 nematodes/g roots for R. similis reported. In Côte d’ivoire, Gnonhouri and al. (2009) showed that since the evolution of nematofauna associated with Cavendish banana cultivation and characterized by the prevalence of P. coffeae, banana orchards are subject to frequent replanting.

 

Incidence of the cohabitation of R. similis and P. coffeae on the multiplication rates of nematodes

 

In general, R. similis accounted for 5 to 28 % of the final population and P. coffeae 72 to 95 % in dixenic inoculation, whether the latter was more abundant in the initial population or not (Table 6). With an initial density of R. similis equal or higher than that on P. coffeae, the final population of the former never exceeded 30 % under the plantain hybrids and cultivar. The final population of nematodes in dixenic inoculation was dominated by P. coffeae (72 to 95 %) in the five initial inoculum densities tested (Table 6). These results account further for the qualitative and quantitative changes that have been recorded with P. coffeae in the nematofauna associated with of banana and plantain cultivation in Côte d’ivoire (Gnonhouri and al. 2009; Sarah, 1989 and Adiko and N'guessan, 2001). The great multiplication and damaging capacity of P. coffeae requires the adoption of the new threshold of 5 nematodes per gram of roots for the implementation the warning system and the development of other integrated control measures for the management of parasitic nematodes of banana and plantain.

 

 

 

 

 

 

 

CONCLUSION

 

The study of the host status of tetraploid plantain FHIA 21 and PITA 3 demonstrated that the former was resistant  to R. similis. Relatively to P. coffeae instead, FHIA 21 appeared as a good host. Like the reference cultivar HORNE 1, PITA 3 appeared susceptible to both nematode species.  Assessment of the incidence of increasing initial density levels of R. similis and P. coffeae lead to the establishment of a damage threshold of 5 P. coffeae per gram of roots, and the confirmation of Guerout’s threshold for R. similis, 10 nematodes per gram of roots. P. coffeae appeared also more aggressive than the banana nematode as in dixenic inoculation, its population prevailed over that R. similis whatever the initial density level. Due to the recomposing of nematode fauna under banana and plantain in Côte d’Ivoire, the new damage threshold of 5 nematodes/gr of root should be considered for the management of plant-parasitic nematodes associated with these crops.

 

 

ACKNOWLEDGEMENTS

 

The study here reported was supported financially by the Fonds Interprofessionnel pour la Recherche et le Conseil Agricoles of Côte d’Ivoire (FIRCA), on behalf of the World Bank and the Government of Côte d’Ivoire, in the framework of the West Agricultural Productivity Program (WAAPP) of the ECOWAS.

 

 

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Cite this Article: Vawa OST, Adiko A, Gnonhouri GP, Otchoumou A (2016). Host Status of Plantain Hybrids FHIA 21 and PITA 3 for Populations of Radopholus similis and Pratylenchus coffeae in Côte D’ivoire. Greener Journal of Agricultural Sciences, 6(9): 262-271, http://doi.org/10.15580/GJAS.2016.9.101416162.