2019-03-30T00:00:00In this study, the effects of culture media, temperature and light and darkness on mycelial growth of L. theobromae were evaluated. Results of potato dextrose agar (PDA) and potato dextrose agar stem exudates (PDASE) were found suitable for mycelial growth of the L. theobromae. The fungus grew from 20-40oC with optimum growth observed at 25-35oC on both media. On the 10th day, mycelial growth at 25-35oC was (15.6mm ± 0.02 - 30.6mm ± 0.05; 18.4mm ± 0.28 - 32.5mm ± 0.10) while mycelial growth recorded with PDASE was (18.2mm ± 0.23 – 31.0mm ± 0.25; 22.8mm ± 0.02 – 38.2mm ± 0.40). There were no significant effects of light and darkness on the mycelial difference (P≤ 0.05) on the growth of L. theobromae. It is recommended from this research work that amended potato dextrose agar (PDA) with stem exudates promoted the growth of test fungus L. theobromae and hence should be used as culture medium for fungi at 35oC.