Effects of gibberellic acid (GA3), 6-benzylaminopurine (BAP) and potassium nitrate (KNO3) on the seed germination and DNA concentration of cucumber (Cucumis sativus) radicle were assessed. The concentrations of these growth stimulants were 0 mM, 1 mM, 5 mM and 10 mM. The cucumber seeds were surface sterilized in ethanol for 5 minutes and rinsed with distilled water before pretreatment with these growth stimulants. The germination study was allowed to stand for 14 days and DNA concentration of cucumber radicle with the highest germination count was determined for each growth stimulant. The study showed that cucumber seeds had higher germination count under the light condition than in the dark condition. However, it is not statistically different. The study also showed that percentage germination of cucumber seeds was enhanced by GA3 (57 – 72%) and BAP (62 – 70%) when compared to the Control (50%) except KNO3 (41 – 44%). Across the treatments, GA3 gave the highest germination percentage followed by BAP with 5 mM concentration producing the highest germination count while 10 mM recorded the highest in KNO3. The DNA concentration of the cucumber radicle that produced these highest germination percentage are: GA3 (47.40 ng/µl), BAP (98.87 ng/µl), KNO3 (103.23 ng/µl) and Control (79.73 ng/µl). The analysis of variance (ANOVA) showed that treatments are significant at p-value (0.0001) < 5% significant level for cucumber seed. The study recommends the use of 5 mM GA3 in germinating cucumber seeds.