Identification of fungal pathogens associated with mango disease in Usmanu Danfodiyo University, Sokoto was carried out in selected Areas of the University, with the aim to Isolate and identify the fungi associated with fruits, leaves and stem, and genotypically characterize the most significantly occurred fungal isolates by PCR assays. Mango trees were selected from four different locations; there are about eight different symptoms associated with the diseases of mango trees in the study site. These include; gum exudation, bark splitting, discoloration and darkening of the bark, wilting of leaves, root decay, whitish substance on and around the root surfaces and wilting of branches. Based on morphological identification, 448 fungal isolates were tentatively identified into 7 genera and 11 species, namely Alternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, Colletotrichum gloeosporioides, Fusarium semitectum, Papulaspora sp., Penicillium sp., Pestalotiopsis guepinii and Rhizopus stolonifer among others. The extracted DNA from the most frequent selected isolates of fungal species was successfully amplified with universal primer ITS1 and ITS4 primer, sequencing confirmed the identification of 7 species of fungi from gel amplicons with 99-100% similarities index. The present study showed that diverse groups of fungal genera were associated with leaf spot of mango. The F. verticillioides and L. theobromae were found to be associated with anthracnose of mango, implying that other fungal species may act in synergy with C. gloeosporioides to cause fungal disease of mango in the study areas. The study recommends awareness creation on good orchard management practices, regular monitoring of the orchards by the management and establishing a link with an expert for advice.