Rice (Oryza sativa L.) dirty grains and blighted panicles symptoms were observed on about 1-5% of plants within farmers’ fields in Essequibo (region#2,3), Demerara (reg.#4,5) and Berbice (reg.#6) in Guyana. Four samples at maturity stage with Dirty Discolored Grains of Paddy and Panicle Blight were harvested, labeled and place in paper bags in March 2022 from regions#2, 3, 4 and 5 for diagnosis and molecular confirmation. Initial identification to genus-level was done using morphological features and MALDI-ToF. Results of the morphological and molecular sequencing identifies fungal isolates Curvularia sp., with top matches of >99% for C. lunata, C. dactyloctenicola, and >98% for C. inaequalis. Similarly, sequencing analysis identified Pantoea sp. with matches of >99% to several closely related species [including P. stewartia P. allii, P. ananatis, P. agglomerans and P. pleuroti] and Pseudomonas oryzihabitans with top matches of >99% (European Molecular Biology Laboratory (EMBL) database via the European Bioinformatics Institute (EBI), (CABI, 2023). Pathogenicity tests were carried out following Koch’s postulate and confirmed that Curvularia sp., Pantoea sp., and Pseudomonas oryzihabitans were the pathogens responsible for dirty discolored grains of paddy and panicle blight symptoms observed, while the control plants remained healthy and symptomless. To our knowledge, this is the first report that identifies and confirm these 3 pathogens working together and causing the dirty discolored grains of paddy and blighted panicle symptoms in Guyana.