Greener Journal of Biological Sciences
Vol. 9(2), pp. 22-28, 2019
Copyright ©2019, the copyright of this article is retained by the author(s)
Antagonistic effect of developed probiotic yoghurt against some selected food-borne pathogens during cold storage
* 1Aforijiku, S., 2Onilude, A.A. and 3Wakil, S.M.
1 Department of Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria.
2 Department of Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria.
3 Department of Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria.
Article No.: 071719137
This study was carried out to investigate the antagonistic effect of probiotic yoghurt against some selected food-borne pathogens during cold storage. Probiotic yoghurt was produced from processed cow milk using controlled fermentation, and later inoculated with selected food-borne pathogens at inoculum level of 105 CFU/mL. The antagonistic effect of the probiotic yoghurt against the food-borne pathogens during cold storage (4oC) for 2 days was studied using standard methods. The results obtained demonstrated that the probiotic yoghurt inhibited growth of food-borne pathogens including Salmonella typhimurium ATCC13311, Proteus mirabilis ATCC25933, and Escherichia coli ATCC2592 in the yoghurt within 24 hr. Their counts decreased from 105-102 CFU/mL, with pH and probiotic counts ranging between 4.48-4.35, and 106-108 CFU/mL, respectively. The probiotic bacteria have the ability to suppress the growth of pathogen like E. coli in yoghurt. This inhibitory effect may be due to low pH of yoghurt. The probiotic cultures in the yoghurt can be used as biopreservatives in food and pharmaceutical industries.
E-mail: dayophd@ yahoo. com
Food-borne pathogens; probiotic yoghurt; antagonistic effect; inoculum level
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Al-Delaimy, K. S. and Hamamdeh, Y. M. (2013). Inhibition of Staphylococcus aureus by lactic acid bacteria and /or Bifidobacterium lactis during milk fermentation and storage. J. Microbio. Biotechnol. Food Sci. 2(4): 2406-2419.
APHA, (2004). Standards Methods for the examination of dairy products.17thEdn. American Public Health Association Inc, Washington, DC,USA.
Bachrouri, M., Quinto, E. J. and Mora, M.T. (2006). Survival of Escherichia coli O157:H7 during storage of yoghurt at different temperatures. J. Food Sci. 67 (5):1899-1903
Bibbal, D., Kerouredan, M., Loukiadis, E., Scheutz, F., Oswald, E. and Brugere, H. (2014). Slaughterhouse effluent discharges into rivers not responsible for environmental occurrence of enteroaggregative Escherichia coli. Veter. Microbio.168: 451-45.
Brant, R. J. and Todd, R. K. (2014). Impact of genomics on the field of probiotic research: historical perspectives to modern paradigms. Tot. Env. 106:141-156.
CDC, (2016)." Diarrhea: Common Illness, Centers for Disease Control and Prevention. Atlanta, USA, Pp.4
Donkor, O. N., Henriksson, A., Vasiljevic, T. and Shah, N. P. (2006). Effect of acidification on the activity of probiotics in yoghurt during cold storage. Internet. Dairy. J. 16: 1181-1189.
Evariste, B., Assèta, K., KuanAbdoulaye, T., Touwendsida, S. B., Hadiza, B. I., Soutongnooma, C. B., Isidore, J., Ouindgueta, B., Saidou, K., Cheikna, Z., Alfred, S. T. and Nicolas, B. (2017). Characterization of Diarrheagenic Escherichiacoli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry)from Cattle’s Markets in Ouagadougou, Burkina Faso. Int. J. Environ. Res. Public Health.14: 1100
Gopalakrishnan,V. (2018).The influence of the gut microbiome on cancer, immunity and cancer immunotherapy, and Cancer cell. Sci. Am. 33(4) 570-580
IS0. (2003).Yoghurt: Enumeration of characteristics microorganisms by colony count technique at 37 0C. IstEdn., International Standard Organisation, Brussels, Belgium.
ISO. (2004). Horizontal Method for Detection and Enumeration of L. monocytogenes. 2ndEdn, International Standard Organisation, Brussels, Belgium.
Kasimoglu, A. And Akgun, S. (2004). Survival of E. coli O157:H7 in the processing and post-processing stages of acidophilus yoghurt. Internet. J. Food Sci.Technol. 39: 563-568.
Mohammed, S., Ahlgren, J. A. and Horne, D. (2016). Structural characterization and biological activities of an exopolysaccharide kefir produced by Lactobacillus kefiranofaciens WT-2B(T). J. Agric. Food Chem. 52: 5533-5538.
Nassib, T. A., El- din, M. Z. and El- Sharoud, W. M. (2006). Effect of thermophilic lactic acid bacteria on the viability of Salmonella serovartyphimurium PT8 during milk fermentation and preparation of buffalo’s yoghurt. Internet. Dairy. Technol. 59:29-34.
Nikolic, M., Terzic-Vidojevic, A., Jovcic, B., Begovic, Golic, N. and Topisirovic, L. (2008). Characterization of lactic acid bacteria isolated from Bukuljac, a homemade goat’s milk cheese. Internet. J. Food Microbio.122:162-170.
Rahmann, M., Gul, S. and Farooqi, W. (1999). Selection of starter culture for yoghurt preparation and its Antibacterial of Activity. Pak. J. Bio sci. 2: 131-133.
Rebello, R. C. and Regua-Mangia, A. H. (2014). Potential enterovirulence and antimicrobial resistance in Escherichia coli isolates from aquatic environments in Rio de Janeiro, Brazilian Science. Tot. Env. 490 :19–27
Ting, T. L. and X, Z. (2019). Lactobacillus spp as probiotics for prevention and treatments of enteritis in the lined seashore. Sci. Am. 503:16-25
Tribe, I. G., Cowell, N. D., Cameron, Peter.and Cameron, S. (2014). "An outbreak of Salmonella typhimurium phage type 135 infection linked to the consumption of raw shell eggs in an aged care facility". Communi. Dis. Intelli. 26 (1): 38–90.
Tsegaye, M. and Ashenafi, M. (2005). Fate of Escherichia coli O157:H7 during the processing and storage of Ergo and Ayib, traditional Ethiopian dairy products. Internet. J. Food Microbio. 103: 325-334
Wang ,Y. K., Li., S. N, Lu, C. S, Peing, D. S., Su, Y. C, Wu, D. C., Jan, C. M., Lai, C. H., Wang, T. N. and Wang, W. M. (2004). Effect of injecting Lactobacillus and Bifidobacterium containing yoghurt in subjects with colonized Helicobacter pylori. Am. J. Clin. Nutr. 80(3): 737-4.
Cite this Article: Aforijiku, S; Onilude, AA; Wakil, SM (2019). Antagonistic effect of developed probiotic yoghurt against some selected food-borne pathogens during cold storage. Greener Journal of Biological Sciences, 9(2): 22-28, http://doi.org/10.15580/GJBS.2019.2.071719137.
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