Greener Journal of Biological Sciences Vol. 9(2), pp. 29-34, 2019 ISSN: 2276-7762 Copyright ©2019, the copyright of this article is retained by the author(s) DOI Link: http://doi.org/10.15580/GJBS.2019.2.090119163 https://gjournals.org/GJBS |
Detection of Aethina tumida Murray 1867 (Coleoptera: Nitidulidae) and the yeast Kodamaea ohmeri in Africanized honey bees from southeastern of Brazil
1 Edlene Ribeiro Prudêncio; 2 Geraldo dos Santos Oliveira; 3Maria Cristina Affonso Lorenzon*; 3 Alexandre dos Santos Nogueira; 1 Rosa Helena Luchese
1Food Microbiology Laboratory, Departmentof Food Technology, Federal Rural University of Rio de Janeiro, 23897-970 Seropédica, Rio de Janeiro, Brazil.
2 Fernandes Figueira Institute – Fiocruz; Av. Rui Barbosa 716, 22.250-020, Rio de Janeiro, RJ, Brazil.
3 Animal Production Department, Universidade Federal Rural do Rio de Janeiro, CEP 23897-000, Seropédica, Rio de Janeiro, Brazil.
ARTICLE INFO |
ABSTRACT |
Article No.: 090119163 Type: Research DOI: 10.15580/GJBS.2019.2.090119163
|
The small hive beetle (SHB), is a significant pest of honeybee (Apis mellifera L.) hives in various regions throughout the world, including the southeastern Brazil. Samples of honeycombs and pollen, bees and SHB were collected from two apiaries located nearby Rio de Janeiro city, southeastern Brazil. Samples were homogenized and plated in Sabouraud agar. White, moist, creamy colonies were counted and identified phenotyphically as Kodamaea ohmeri. At the time the sampling was done, the yeast contamination was low in all parts of the hives but, yet above the Brazilian legal standards for honey, which established a maximum of 102 CFU g-1 of mold and yeasts. |
Submitted: 01/09/2019 Accepted: 03/09/2019 Published: 26/09/2019 |
|
*Corresponding Author Maria CA Lorenzon E-mail: affonsoneta@ gmail. com Phone: +55213787 3975 |
|
Keywords: honeybee; symbiosis; small beetle; beekeeping; Microbiology |
|
|
|
Return to Content View [Full Article - PDF] | Post-Publication Peer-review Rundown View/get involved, click [Peer-review] |
REFERENCES
Aidoo KE, Rob Nout MJ and Sarkar PK (2006). Occurrence and function of yeasts in Asian indigenous fermented foods. FEMS Yeast Res.6(1): 30–39.
Al Toufailia H, Alves DA, Bená DDC, Bento JMS, Iwanicki NSA, Cline AR, et al. (2017). First record of small hive beetle, Aethina tumida Murray, in South America. J. Apic. Res.56(1): 76–80.
Amos BA, Hayes RA, Leemon DM and Furlong MJ (2019). Small Hive Beetle (Coleoptera: Nitidulidae) and the Yeast, Kodamaea ohmeri: A Facultative Relationship under Laboratory Conditions. J. Econ. Entomol.112(2): 515–524.
Amos BA, Leemon DL, Hayes RA, Cribb BW, Furlong MJ. 2018. Associations between the small hive beetle and the yeast Kodamaea ohmeri throughout the host life cycle .Journal of Economic Entomology, 111 (4):1501–1508 Benda ND, Boucias D, Torto B and Teal P (2008). Detection and characterization of kodamaea ohmeri associated with small hive beetle Aethina tumida infesting honey bee hives. J. Apic. Res.47(3): 194–201.
Brasil (2000) Instrução Normativa Nº 11. Regulamento Técnico de Identidade e Qualidade do Mel. Ministério da Agricultura, Departamento de Inspeção de Produtos de Origem Animal – DIPOA. Diário Oficial da União 23/10/00, Seção I, págs. 16-17.
Cassey P, Blackburn TIMM, Duncan RP and Chown SL (2005). Concerning invasive species : Reply to Brown and Sax. Biol. Invasions 30: 475–480.
Conklin TM. 2012. Investigations of small hive beetle-yeast associations Thesis. Pennsylvania State University
Cordeiro EMG, Soares PL, Alves DA and Corrêa AS (2019). Updating the saga of the small hive beetle (Aethina tumida): molecular inference of the origin of the South American invasion. Apidologie: 273–276.
Cuthbertson AGS and Brown MA (2009). Issues affecting British honey bee biodiversity and the need for conservation of this important ecological component. Int. J. Environ. Sci. Technol.6(4): 695–699.
Cuthbertson AGS, Wakefield ME, Powell ME, Marris G, Anderson H, Budge GE, et al. (2013). The small hive beetle Aethina tumida: A review of its biology and control measures. Curr. Zool. 59(5): 644–653.
Deveza MV, Keller KM, Lorenzon MCA, Nunes LMT, Sales ÉO and Barth OM (2015). Mycotoxicological and palynological profiles of commercial brands of dried bee pollen. Brazilian J. Microbiol.46(4): 1171–1176.
Guerra JCV, Gonçalves LS and De Jong D (2000). Africanized honey bees (Apis mellifera L.) are more efficient at removing worker brood artificially infested with the parasitic mite Varroa jacobsoni Oudemans than are Italian bees or Italian/Africanized hybrids. Genet. Mol. Biol.23(1): 89–92.
Hayes RA, Rice SJ, Amos BA and Leemon DM (2015). Increased attractiveness of honeybee hive product volatiles to adult small hive beetle, Aethina tumida, resulting from small hive beetle larval infestation. Entomol. Exp. Appl.155(3): 240–248.
Lamei S, Stephan JG, Riesbeck K, Vasquez A, Olofsson T, Nilson B, et al. (2019). The secretome of honey bee-specific lactic acid bacteria inhibits Paenibacillus larvae growth. J. Apic. Res.58(3): 405–412.
Lorenzon, M.C; Tassinari, W.S.; Koshiyama, A.S.; Almeida, C.T. 2012. (Beekeeping indicators and challenges – A Brazilian Portrait). 1. ed. Espírito Santo: ABOVE, 272p.
Lorenzon MCA, Resende M, Lyssei E, ADAB . 2019. (The small hive beetle Aethina tumida invasor of Africanized honeybees comes in the southeastern of Brazil). Animal Agrobusiness Brazil. https://animalbusiness.com.br/universidade/o-pequeno-besouro-invasor-das-abelhas-meliferaschega-a-regiao-sudeste/
Lorenzon MCA (2017). [Quality control and Beekeeping Sanity]. Defesa Agropecuári, Brazil. Retrieved from: https://rebecacaroliny.wixsite.com/inovadefesa/inicio/livro-defesa-da-qualidade-do-mel-e-da-sanidade-ap%C3%ADcola-saiba-porque-as-abelhas-est%C3%A3o-em-risco.
Lounsberry Z, Spiewok S, Pernal SF, Sonstegard TS, Hood WM, Pettis J, et al. (2010). Worldwide Diaspora of Aethina tumida (Coleoptera: Nitidulidae), a Nest Parasite of Honey Bees. Ann. Entomol. Soc. Am.103(4): 671–677.
McGlashan K. 2011. Examination into the relationship between Kodamaeaohmeri and the small hive beetle Aethina tumida. Master of Science. The University of Queensland, Brisbane
Mutinelli F (2011). The spread of pathogens through trade in honey bees and their products (including queen bees and semen): overview and recent developments. Rev. Sci. Tech. l’OIE30(1): 257–271.
Mycobank (2019). Mycobank. (consulted http://www.mycobank.org/name/Kodamaea ohmeri)
Neumann P and Elzen PJ (2004). The biology of the small hive beetle (Aethina tumida, Coleoptera: Nitidulidae): Gaps in our knowledge of an invasive species. Apidologie35: 229–247.
Olofsson TC, Butler E, Markowicz P, Lindholm C, Larsson L and Vásquez A (2014). Lactic acid bacterial symbionts in honeybees - an unknown key to honey’s antimicrobial and therapeutic activities. Int. Wound J.: 1–12.
Shang ST, Lin JC, Ho SJ, Yang YS, Chang FY and Wang NC (2010). The Emerging Life-threatening Opportunistic Fungal Pathogen Kodamaea ohmeri: Optimal Treatment and Literature Review. J. Microbiol. Immunol. Infect. 43(3): 200–206.
Sidrim JJC and Moreira JLB (1999). Fundamentos clínicos e laboratoriais da micologia médica.
Spiewok S, Pettis JS, Duncan M, Spooner-Hart R, Westervelt D and Neumann P (2007). Small hive beetle, Aethina tumida , populations I: Infestation levels of honeybee colonies, apiaries and regions. Apidologie 38(6): 595–605.
Spivak M (1996). Honey bee hygienic behavior and defense against Varroa jacobsoni Marla. Apidologie 27: 245–260.
Teal P (2006). An in-hive trap and attractant composition for the control of the small hive beetle, Aethina tumida. p.: 10.
Teixeira ÉW, De Jong D, Sattler A- and Message D (2016). Aethina tumida Murray (Coleoptera, nitidulidae), o pequeno besouro das colmeias, chega ao Brasil. Mensagem Doce136: 2–10.
Torto B, Boucias DG, Arbogast RT, Tumlinson JH and Teal PEA (2007). Multitrophic interaction facilitates parasite-host relationship between an invasive beetle and the honey bee. Proc. Natl. Acad. Sci.104(20): 8374–8378.
Cite this Article: Prudêncio ER; Oliveira GS; Lorenzon MCA; Nogueira AS; Luchese RH (2019). Detection of Aethina tumida Murray 1867 (Coleoptera: Nitidulidae) and the yeast Kodamaea ohmeri in Africanized honey bees from southeastern of Brazil. Greener Journal of Biological Sciences, 9(2): 29-34, https://doi.org/10.15580/GJBS.2019.2.090119163. |
Call for Papers
Call for Scholarly Articles
Authors from around the world are invited to send scholarly articles that suits the scope of this journal. The journal is currently open to submissions and will process and publish articles promptly.
The journal is centered on quality and goes about its processes in a very timely fashion. Seasoned editors/reviewers will be consulted to review each article(s), profer quality evaluations and polish the articles with expertise before publication.
Simply send your article(s) as an e-mail attachment to gjbs@acad.gjournals.org or manuscripts.igj@gmail.com.