2020-04-30T00:00:00Introduction: Medical waste includes all types of waste with potential characteristics to spread diseases, generated from healthcare institutions. Objective: The study was carried out to assess the status and challenges of medical waste management practices in Delta State University Teaching Health Institution. Methodology: A cross-sectional survey was carried out between March and August 2019. Multistage sampling approach comprising of purposive sampling and case study approach was employed for the study. The approach involved estimating the quantity of medical waste generated and the evaluation of waste management strategy used by the healthcare institution. A total of 240 respondents were sampled. Collected data were subjected to statistical analysis using SPSS version 21. Result and discussion: Average total weight of waste generated from the departments was estimated to be 948.366 kg/day. Medical waste generation rate was 13.598 kg/patient/day with an average bed per day generation rate of 1.133 kg/bed/day. Kitchen department had the highest generation rate of (254.448 kg/day) whereas the least generation rate was from NET (9.11 kg/day). It was observed that segregation of waste at source exist but poorly implemented and monitored as medical waste was still being mixed and dumped with general wastes that were collected, transported and disposed. Segregation was not carried out in line with NHCWMP standards. Waste generated were collected on a daily base and transported to a designated place for temporary storage. Lidded plastic container, storage house, hand cart, waste skip and wheeled trolley were mainly used to store and transport waste. The institution lack sufficient waste containers to handle volumes of medical waste. The result revealed that higher percentage of waste handlers were poorly educated and irrespective of the availability of PPE; they were observed to be wrongly used by waste handlers. Land fill was revealed to be the most preferred final disposal option but occasionally incineration and open pit burning were also adopted. Conclusion: This study has helped to establish a baseline data on medical waste management strategy in Delta State University Teaching Health Institution.