2020-06-28T00:00:00The role of Aspergillus Species in cases of food borne infection have been established and reported to have substantial degree of morbidity and mortality among immunoincompetent individuals. This study was carried out to determine the pathogenicity of Aspergillus species isolated from bean seed samples. A total of 90 representative bean seed samples of different varieties were randomly collected from different shops and open markets in Ihiala Local Government Area, Anambra State, and screened for the presence of Aspergillus species. The isolates obtained were characterized and identified using macroscopic, microscopic and molecular characteristics. The pathogenic potentials of the isolates on immunocompetent and immunoincompetent mice were investigated by oral administration of 0.5 ml of the inoculum (108cells/ml) into the mice. The mice were kept under complete observation for 4 weeks for obvious pathological features and mortalities. It was observed that 32 (35.56 %) and 14 (14.44 %) of the studied bean seed samples recorded Aspergillus species for surface and internal contaminants. Oloka bean seeds (70 %/30 %) were most contaminated. Aspergillus flavus strain HUS6 (AFHUS6), Aspergillus niger strain HG48 (ANHG48), Aspergillus niger strain HUS1 (ANHUS1), Aspergillus tubingiensis strains EM-CN1 (ATEM-CN1), Aspergillus aculeatus strain AN5 (AAAN5) and Aspergillus awamori strain DN-SN2 (AW DN-SN2) were isolated from the bean seed samples, of which AN HUS1 was mostly seen in the studied samples There were significant (P<0.05) obvious pathological features and lesion on the internal organs, majorly the lungs of the infected immunoincompetent mice, and the pathological features of those mice infected with AFHUS6 and ANHUS1 were most pronounced. There were significant (P<0.05) mean plate counts of the test isolates from the organs of immunoincompetent mice, and decreased in organ weight to body weight ratio. Thus, this study has shown that the fungi isolates showed obvious pathological features majorly on infected immunoincompetent mice, of which AFHUS6 was most pathogenic.