2020-06-28T00:00:00Ascertaining the real characteristics, identity and prevalence of microorganism is the major step in controlling foodborne pathogens. This study was carried out to determine the identities and prevalence of Aspergillus species associated with bean seed samples sold in Ihiala, Anambra State. A total of 90 representative bean seed samples of different varieties (mesugar, Oloka, honey, potasco white, potasco brown, Sokoto bean, Iron white, Iron brown and soya bean) were randomly collected from different shops and open markets in Ihiala Local Government Area, Anambra State, and screened for the presence of Aspergillus species using spread plate technique and surface plating of 25 seeds per Petri dish on sterile poured plates. The isolates obtained were characterized and identified using macroscopic, microscopic and molecular characteristics. It was observed that 32 (35.56 %) and 14 (14.44 %) of the studied bean seed samples recorded Aspergillus species for surface and internal contaminants. The surface of Oloka bean seeds (70 %/30 %) and inner part of Sokoto bean seeds (50%/50%) were most contaminated. Aspergillus flavus strain HUS6 (AFHUS6), Aspergillus niger strain HG48 (ANHG48), Aspergillus niger strain HUS1 (ANHUS1), Aspergillus tubingiensis strains EM-CN1 (ATEM-CN1), Aspergillus aculeatus strain AN5 (AAAN5) and Aspergillus awamori strain DN-SN2 (AW DN-SN2) were isolated from the bean seed samples. AN HUS1 was mostly significant (P<0.05) in both surface and internal contamination of the studies bean seed sample. Thus, this study has shown that AFHUS6, ANHG48, ANHUS1, ATEM-CN1, AAAN5and AW DN-SN2 were isolated from the studied bean seed samples of which ANHUS1 was most prevalence mostly on the surface of Oloka bean and internally in Sokoto bean.