Background: Cervical cancer is the fourth most frequent cancer in women globally. About 90% of its burden occur in low- and middle-income countries. It is preventable through screening. Knowledge and acceptance of cervical cancer screening among women of reproductive age is low in developing countries. This study aimed at assessing knowledge, perception, and acceptability of cervical cancer screening among women of reproductive age in Ikwerre Local Government Area. Methodology: A total of 213 women of reproductive age participated and provided information on their biodata, reproductive history, knowledge of cervical cancer, its screening, perception, and acceptability. Data was analyzed using SPSS version 22 and results were presented in frequency tables. Bivariate analysis was done using Chi (X2) square test (P < 0.05). Results: The mean age was 30.3 ± 7.9 years, 84 (39.4%) had knowledge of cervical cancer, 13 (6.1%) had very good knowledge of cervical cancer screening, 127 (59.6%) had a positive perception, and 156 (81.7%) were willing to accept cervical cancer screening. Knowledge of cervical cancer screening was associated with the level of education of respondents (X2 = 43.661; P <0.0001) and their sources of information about cervical cancer (P < 0.00001). Conclusion: The knowledge of cervical cancer as well as its screening among women of reproductive age is poor while positive perception and the acceptability of cervical cancer screening were high. There is need for more health education programs on cervical cancer screening in the rural areas to increase the level of knowledge of cervical cancer screening.