Onubeze and Oguwike
Greener Journal of Medical Sciences Vol. 6 (2), pp. 030-035, May 2016.
Manuscript Number: 041316077
Evaluation of Activities of Aquoepus Extract of M Avocado (Persea americana) on Wound Healing and Biochemical Indices of Albino Wistar Rats
Onubeze DPM1 and Oguwike FN2*
1Department of Community Medicine, Anambra State University Teaching Hospital Amaku Awka, Nigeria.
2Department of Physiology, Faculty of Basic Medical Sciences Anambra State University Uli, Nigeria.
The aqueous crude extracts of some plants could play a helpful role in hastening the coagulation system in a damaged tissue or vessels at the same time restore order in a diseased tissue, hence this research evaluated the activities of aqueous crude extract of Avocado (Persae Americana) on wound healing and biochemical profile of male albino wistar rats using Sixty rats. The animals weighing 180-200g were randomly selected and placed into five (5) groups group A (control), group B (Skin ulcered rats on extract), group C (rats with skin ulcers on normal saline), group D (wound incised animals on extract) and group E(incised rats on normal saline). All the test rats were 12 animals in each group. They were gavaged with 1ml of aqueous extracts of Avocado for twenty eight days (acute study) before blood samples were collected from them for investigations.
The results showed that avocado extract decreased haemoglobin concentration, Packed cell volume and White blood cell (p<0.05).It has no effect on platelet counts (p>0.05), it shortened days of healing in rats inflicted with wounds and those with skin ulcers. The bleeding and clotting times were reduced as well as the cholesterol level (p<0.05), again the LD50 of Avocado is zero in albino wistar rats.
It can be deduced from the study that Avocado (Persae Americana) possess some active principles that hastens wound healing and reduces cholesterol level.
Key words: Wound healing, avocado, bleeding time, clotting time, cholesterol hemoglobin.
Alexander R.R and Griffit J.M (1993). Haematorit determination by the cyanmethamoglobin method in Biomedical methods 2nd ed. John Wiley and sons Inc. Publications, New York pp 186-197.
Ab Avocado history” index fresh avocado. Bloomington, CA: index fresh avocado 2007. Archived from the original on Dec 25 2007. Retrieved 2007-12-29.
Baker F.J, Silverton R.E (1985). Investigation for haemostatic abnormalities. Introduction t medical laboratory technology. pp 148 Butterworth’s publication Woburn London.
Constable B.J (1963). Changes in blood volume and Prothrombin time of rat and guniea pigs from birth to maturity: J. physiology (London) 197:229-238.
Dacie J.V and Lewis S.M (1975). Practical haematology 5th ed. Churchill Livingstone London pp 502-503.
Dejana E, Villa S, De Galactano G (1982). Bleeding time of rats, a comparison of different experimental conditions. Thromb: haemostat. 48:108-111.
Ding H, Ambrosio S.M, Han C, Pan L, Kinghorn A.D. (2011). Aliphatic acetoginin constituent of avocado fruits inhibits human oral cancer cell proliferation by targeting the EGER/RAS/RHE/MEK/ERK1/2 pathway Biochem biophys Res common 409(3): 465-9.
Harbourne J.B.C, (1973): Phytochemical, methods Chpman and Hall, London p226.
Ita S.O, Etim O.E, Ben E.E, Ekpo O.F (2007). Haematopoietic properties of ethanolic leaf extract of Ageratum conyzoides (goat weed) in albino rats. Nigerian Journal of Physiological Sciences 22(1-2) 83-87.
Lawrence W.T, Diegelmann R.F (1994). Growth factors in wound healing. CLin. Dermatol 12:157.
Ligha A.E, Fawehinmi H.B (2008). Histological observations of the wound healing properties of plant exudates of Jatropha cucas Linn. Journal of Experimental and clinical anatomy 7 (1) 17-21.
Lorke D (1983). A new approach to practical acute to toxicity tests Arch. Toxicity testing Arch. Toxical 54:275-278.
Prockop D.J, Kiririkko K.J (1995). Collagen, molecular biology, diseases and potential for therapy. Annual Rev. Biochem 64:403.
Wikipedia Foundation Inc June (2010).
Zeides B.C, Leah A (2010). “Eat this the Hass” avocado, black and green and creating.
Call for Papers
Call for Scholarly Articles
Authors from around the world are invited to send scholary articles that suits the scope of this journal. The journal is currently open to submissions and will process and publish articles monthly in two yearly issues.
The journal is centered on quality and goes about its processes in a very timely fashion. Seasoned editors/reviewers will be consulted to review each article(s), profer quality evaluations and polish the articles with expertise before publication.
Simply send your article(s) as an e-mail attachment to firstname.lastname@example.org or email@example.com.