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Odume et al
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Greener Journal of Medical Sciences Vol. 11(2), pp. 122-129, 2021 ISSN: 2276-7797 Copyright ©2021, the copyright of this article is retained by the author(s) |
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Diagnostic Accuracy of TB-LAMP for Diagnosis of Pulmonary Tuberculosis among Adult Presumptive TB in Nigeria
1Bethrand ODUME, 1Nkiru NWOKOYE, 2Ineke Spruijt, 2Andrii SLYZKYI, 3Cyril DIM, 1Ogoamaka CHUKWUOG, 1Sani USENI, 1OGBUDEBE Chidubem, 1Ikechukwu ANAEDOBE, 1Peter NWADIKE, 4Emeka ELOM, 5Debby NONGO, 5Rupert ENEOGU, 5Temitayo ODUSOTE, 5Omesalewa OYELARAN, 4Adebola LAWANSON
1KNCV Tuberculosis Foundation Nigeria;
2KNCV Tuberculosis Foundation Netherlands
3Department of Obstetrics & Gynaecology, College of Medicine, University of Nigeria, Ituku-Ozalla
4National Tuberculosis, Leprosy and Buruli ulcer Control Program, Nigeria;
5United States Agency for International Development Nigeria.
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ARTICLE INFO |
ABSTRACT |
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Article No.: 092921097 Type: Research |
Background: Microscopy is still used in resource-limited countries for PTB diagnosis because of the challenges associated with the GeneXpert instrument. For these settings, the WHO recommended the replacement of microscopy with TB-LAMP for PTB diagnosis in adults. Evidence supporting this recommendation shows a wide variation in quality and TB-LAMP diagnostic accuracy; thus, the need to validate the assay before its deployment in Nigeria.
Methods: A cross-sectional study of 2636 consenting eligible adult presumptive TB from health facilities in two States of Nigeria. Sputum specimens were analyzed for PTB using TB-LAMP, Ziehl-Neelsen microscopy, Xpert, and solid culture (reference standard). Sensitivity and specificity of TB-LAMP, Xpert and smear microscopy for PTB diagnosis were determined.
Results: Sensitivity and specificity of TB-LAMP for PTB diagnosis among all participants were 76.7% and 99.3% respectively (Youden Index [J] = 0.76). TB-LAMP’s PTB diagnostic accuracy was higher than microscopy (sensitivity = 54.3%, specificity = 99.8%, J = 0.54), but lower than Xpert (sensitivity = 84.5%, specificity = 99.1%). For HIV-seropositive participants, the diagnostic accuracy of TB-LAMP was similar to microscopy and Xpert (p > 0.05).
Conclusion: TB-LAMP had high accuracy for adult PTB diagnosis in Nigeria, and it can replace microscopy for PTB diagnosis in adults. |
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Accepted: 29/09/2021 Published: 11/10/2021 |
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*Corresponding Author Nkiru Nwokoye E-mail: nnwokoye@ kncvnigeria.org Phone: +23481 3650 1810 |
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Keywords: National Tuberculosis; Leprosy and Buruli Ulcer Control Program; Diagnostic accuracy, AFB microscopy; Xpert MTB/RIF; Solid culture. |
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Cite this Article: Odume B, Nwokoye N, Spruijt I, Slyzkyi A, Dim C, Chukwuog O, Useni S, Ogbudebe C, Anaedobe I, Nwadike P, Elom E, Nongo D, Eneogu R, Odusote T, Oyelaran O, Lawanson A (2021). Diagnostic Accuracy of TB-LAMP for Diagnosis of Pulmonary Tuberculosis among Adult Presumptive TB in Nigeria. Greener Journal of Medical Sciences, 11(2): 122-129.
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