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Isah et al
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Greener Journal of Medical Sciences Vol. 9(2), pp. 40-45, 2019 Copyright ©2019, the copyright of this article is retained by the author(s) DOI Link: https://doi.org/10.15580/GJMS.2019.2.110619199 https://gjournals.org/GJMS |
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Quantitative Phytochemical and In-Vitro Antimicrobial Activity of Aqueous Leaves Extract of Blue Pussy leaf (Nelsonia Canescens) (Lam.) Spreng
1*Isah Hussaini, 1Haruna Yusuf, 1Elinge Cosmos Moki, and 2Bamidele Victor Owoyele
1Department of Pure and Applied Chemistry, Kebbi State University of Science and Technology, Nigeria.
2Department of Physiology, University of Ilorin
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ARTICLE INFO |
ABSTRACT |
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Article No.: 110619199 Type: Research DOI: 10.15580/GJMS.2019.2.110619199
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Currently, there is a renewed interest in drugs of natural origin simply because they are green medicine and green medicine offer safe, effective treatment, with minimal or no side effect, easily availability, cheap and are in great demand in the developed World health care. This study is aimed at evaluating the phytochemical constituents and antimicrobial activity of aqueous leaves extract of blue pussy leaf Nelsonia canescens. Phytochemical analysis, cork borer methods, and Tube dilution methods for MIC and MBC were employed to determine the phytochemical constituents and antimicrobial activity of the aqueous leaves extracts of Nelsonia canescens. Results obtained reveals the presence of alkaloid, total phenol, flavonoids, saponins and tannins at concentration of 330.00±0.00, 144.60±1.76, 107.04±0.62, 72.50±00 and 42.17±1.17 mg/g) respectively. The antibacterial activity of the plant crude extract active on Escherichia coli had the highest mean zone of inhibition of (20.05±0.05) at 20 mg/ml with the MIC at 1.25 mg/ml and MBC of 10 mg/ml. Staphylococcus aurus had a mean zone of inhibition of (18.00±0.00) at 20 mg/ml, with the MIC at 1.25 mg/ml and MBC of 5.0 mg/ml. The plant crude extract was active against Gram-negative bacteria; Pseudomonas aeruginosa, Staphylococcus aurus, Escherichia coli, and Salmonella, typhi. Gram-positive bacteria, Staphylococcus aurus and Streptococcus pyogenes, had wide zones of inhibition when compared to the activity of the control drug. The presence of some of the phytochemicals and antibacterial activity could explain their use traditionally for the treatment of a wide range of illnesses. |
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Submitted: 06/11/2019 Accepted: 08/11/2019 Published: 30/11/2019 |
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*Corresponding Author Isah Hussaini E-mail: hisado100@ gmail. com Phone: +2348035040589 |
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Keywords: Nelsonia canescens; Antimicrobial; Quantitative Phytochemicals; Crude extract; Medicine |
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Cite this Article: Isah, H; Haruna Y; Elinge, CM; and Owoyele BV (2019). Quantitative Phytochemical and In-Vitro Antimicrobial Activity of Aqueous Leaves Extract of Blue Pussy leaf (Nelsonia Canescens) (Lam.) Spreng. Greener Journal of Medical Sciences, 9(2): 40-45, https://doi.org/10.15580/GJMS.2019.2.110619199. |
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