Greener Journal of Physical Sciences

Greener Journal of  Physical Sciences Vol. 2 (1), pp. 020-026, January, 2012.

ISSN: 2276-7851 © 2012 Greener Journals

Research Paper

Manuscript Number: GJPS1205

 

High Performance Liquid Chromatography Assay with UV Detection for the Determination of Ciprofloxacin in Human Plasma

 

*Maged  Alwan Noman¹and Hussein O.kadi ²

 

¹Faculty of Pharmacy, Department of Pharmaceutics, Sana'a University, Yemen,

²Department of pharmacology, Faculty of Medicine, Sana'a University, Yemen,

 

*Corresponding Author Email: magedalwan65 @ gmail.com

Abstract:

Accurate, sensitive and specific isocratic High Performance Liquid Chromatography method with UV detection was developed, validated and applied for the determination of Ciprofloxacin in human plasma. Sample preparation was carried out with the addition of an internal standard Gatifluoxacin to plasma samples followed by a liquid-liquid extraction using perchloric acid. Separation was achieved with Waters symmetry C18, 5µm, 150 x 3.9 mm column using a mixture of potassium phosphate buffer (pH =3.1) : acetonitrile : methanol (72:12:16, v:v:v) in addition to tetrabeutyleammonium hydroxide as an ion pair reagent. The calibration curve for Ciprofloxacin in plasma was linear in the range 0.5-10 µg/mL with r2 = 0.9998. The lower limit of quantitation (LLOQ) and limit of detection (LOD) for Ciprofloxacin in plasma were respectively 0.032 µg/mL and 0.0090 µg/mL.
 Inter and intra-day precessions in the measurements of three different levels QC samples in human plasma were in the range 0.85-2.47%, 0.14-0.24% and 0.10-0.35% respectively.
 Method recovery, short-and long term stabilities were within the acceptable values. The developed method of analysis provided a sensitive and specific assay for Ciprofloxacin and was found to be suitable for the analysis of the drug in human plasma.
 
Keywords: Ciprofloxacin , HPLC, human plasma.

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