Edagbo and Oyetunji
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Greener Journal of Biochemistry and Biotechnology Vol. 6(1), pp. 01-11, 2019 ISSN: 2384-6321 Copyright ©2019, the copyright of this article is retained by the author(s) DOI Link: https://doi.org/10.15580/GJBB.2019.1.070719132 https://gjournals.org/GJBB |
A Comparative Study of Secondary Metabolites, Amino acids and Protein Profiles of the Host – Parasite Plants in the Relationship between the African Mistletoe, Tapinanthus bangwensis [Engl. and K. Krause] Danser and Two of its Host species
Edagbo, David Enuwa1*; Prof. Oyetunji, Olusola Jacob2
1 Plant Genetic Resources Unit, National Centre for Genetic Resources and Biotechnology, Ibadan, Nigeria.
2 Department of Botany, University of Ibadan, Ibadan, Nigeria.
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ARTICLE INFO |
ABSTRACT |
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Article No.: 070719128 Type: Research DOI: 10.15580/GJBB.2019.1.070719132 |
The presence of secondary metabolites, amino acids and sugar alcohol including protein profile in the host-parasite relations of Tapinanthus bangwensis (Tb) on the host plants, Citrus sinensis (Cs) and Irvingia gabonensis (Ig) were evaluated. Secondary metabolites contained in the host-parasite plants was observed for the Citrus-parasite (tannin - 0.35 Tb, 0.21 Cs; phenol - 0.21 Tb, 0.14 Cs; alkaloids - 1.42 Tb, 0.17 Cs g/100g) and Irvingia-parasite (tannin - 0.23 Tb, 0.18 Ig; phenol - 0.19 Tb, 0.15 Ig; alkaloids - 1.34 Tb, 0.57 Ig g/100g) with comparative higher quantities in the parasite. Free amino acids and sugar alcohol contents in the leaves of host plants revealed the constituents of some of the groups in the infested (tyrosine - 12.26 Cs, 14.70 Ig; aspartic acid - 12.21 Cs, 11.23 Ig ng/g) and uninfested (tyrosine - 10.76 Cs,12.93 Ig; aspartic acid - 9.09 Cs, 9.79 Ig ng/g) which indicated significant higher values for the infested. Protein profiling of the Citrus leaves revealed lack of protein at 25.0 kDa band in the infested host. Assessment of metabolites and protein features in the mistletoe-host relationship affirmed presence of similar metabolites in the host-parasite entity while infested hosts had increased free amino acids and there were noticeable variations in protein banding pattern of host plants with mistletoe incidence. |
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Submitted: 07/07/2019 Accepted: 14/07/2019 Published: 29/08/2019 |
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*Corresponding Author Edagbo, David Enuwa E-mail: dedagbo@ yahoo. com |
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Keywords: Tapinanthus bangwensis; Citrus sinensis; Irvingia gabonensis; infested hosts |
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Cite this Article: Edagbo, DE; Oyetunji, OJ (2019). A Comparative Study of Secondary Metabolites, Amino acids and Protein Profiles of the Host – Parasite Plants in the Relationship between the African Mistletoe, Tapinanthus bangwensis [Engl. and K. Krause] Danser and Two of its Host species. Greener Journal of Biochemistry and Biotechnology, 6(1): 01-11, http://doi.org/10.15580/GJBB.2019.1.070719132. |
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