Greener Journal of Biological Sciences

Greener Journal of  Biological Sciences Vol. 3 (5), pp. 172-178, July 2013.

 © 2013 Authors

Research Paper

Manuscript Number: 032113539

Isolation of High Antibiotic Resistant Fecal Bacteria Indicators, Salmonella and VibrioSpecies from Raw Abattoirs Sewage in Peri-Urban Locations of Nairobi, Kenya

¹Nyamboya Rosemary Atieno, *²Okemo Paul Owuor,

³Ombori Omwoyo

¹Email: mcjowi@gmail.com, Tel: +254721301212, ³Email: richardombori @ gmail. com,

Tel: +254751839856

Department of Plant and Microbial Sciences, Kenyatta University, P.O Box 43844-00100,

Nairobi, Kenya.

*Corresponding Author’s Email: paulokemo @ gmail. com, Tel: +254722942072

Abstract:

The research was conducted to determine indicator organisms’ relationship to specific human pathogens and any presence of antibiotic resistance. Isolationof indicator organisms,Salmonella and Vibrio species was carried out using standard laboratory methods. Sensitivity to antibiotics was determined by the agar diffusion technique. The fecal bacteria load was found to be 6.2 x 106, 5.3 x 105, 2.5 x 104, 2.9 x 104, and 5.0 x 106 CFU/100 mL for fecal streptococci and 3.4 x 105, 4.1 x 103, 3.0 x 104, 2.7 x 103and 3.9 x 105MPN/100 mL for fecal coliforms in cattle wastewater, cattle sludge, goat wastewater, sheep wastewater and a mixture of goat and sheep sludge, respectively.Fecal coliforms showed the highest resistance with a mean resistance frequency of 60.8% (±25.2), followed by Salmonella species at 51.5% (±26.6). Vibrio species showed the lowest mean resistance frequency at 41.6% (±24.8). There was however no significant difference (p=0.859) in resistance among Vibrio, Salmonella, FS and FC isolates at p>0.05.There is a likelihood of slaughterhouse animals and bacteria in the intestines of these animals getting exposed to antibiotics to which the bacteria develop resistance which they can pass to human pathogens and environmental flora.

Keywords: Antibiotic resistance, Sewage, Pathogens and Abattoirs.

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