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Greener Journal of Biological Sciences Vol. 10(2), pp. 42-47, 2020 ISSN: 2276-7762 Copyright ©2020, the copyright of this article is retained by the author(s)
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Evaluation of Lead resistance by newly identified Enterobacter cloacae strain AHZ 01 isolated from contaminated soil
Aisami A1*; Mahdi ZA1; Chindo HA1; James IJ; Garba L2
1Department of Biochemistry, Faculty of Sciences, Gombe State University, P.M.B. 027, Gombe, Nigeria.
2Department of Microbiology, Faculty of Science, Gombe State University, P.M.B. 027, Gombe, Nigeria.
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ARTICLE INFO |
ABSTRACT |
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Article No.: 0622520080 Type: Research
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Heavy metal pollution is currently one of the major environmental challenges worldwide. Lead among the leading heavy metal pollutant. Removal of pollutants through physical and chemical method can also generate secondary pollution but bio-removal of metal pollutants from industrial soil and wastewater by microbes resistant to heavy metal is a fundamental aspect of environmental biotechnology. In this current study, a bacterial was isolated from contaminated soil by using nutrient broth incorporated with Pb(NO3)2. The isolate was identified by gram staining and molecular approach and the effect of lead concentration and temperatures were determined. The isolate is a gram-positive and identified as Enterobacter cloacae strain AHZ 01. The isolates were subjected to different Pb(NO3)2, the results revealed that 85 % of the 50 ppm was removed within 96 hours. The result also showed that 75 %, 67 %and 50 % of 100, 150, and 200 ppm respectively were removed within 96 hours. While for 250 ppm only 20 % was removed after 96 hours, but 250 ppm showed an inhibitory effect with a slower growth rate and less than 20 % was removed. A 35 ℃ was the best temperature for the removal of the Lead ions while 40 and 45 ℃. The r RNA sequence of the isolate was deposited at NCBI Genbank and accession number of MN880497 and the bacterial can remove 85 % of 50 ppm Pb(NO3)2. The isolate is a good candidate for further bioremediation studies. |
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Accepted: 25/06/2020 Published: 28/07/2020 |
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*Corresponding Author Dr Abubakar Aisami E-mail: Abubakar.aisami05@ gmail.com Phone: +234806775052 |
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Keywords: Heavy metals; Lead; rRNA; Enterobacter cloacae; pollution . |
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Cite this Article: Aisami, A; Mahdi, ZA; Chindo, HA; James, IJ; Garba L (2020). Evaluation of Lead resistance by newly identified Enterobacter cloacae strain AHZ 01 isolated from contaminated soil. Greener Journal of Biological Sciences, 10(2): 42-47. |
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