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Enyinnaya et al

Greener Journal of Medical Sciences

Vol. 11(2), pp. 181-186,  2021

ISSN: 2276-7797

Copyright ©2021, the copyright of this article is retained by the author(s)

https://gjournals.org/GJMS

 

 

 

 

Molecular Detection and Antibiotic Susceptibility Profile of ESBL-producing Klebsiella pneumoniae Isolates in a Central Nigerian Tertiary Hospital

 

 

Enyinnaya S.O1*, Iregbu K.C2, Uwaezuoke N.S3, Abdullahi N3, Lawson S.D1

 

 

1Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Sciences, Rivers State University, Port Harcourt, Rivers State, Nigeria.

2Department of Medical Microbiology and Parasitology, National Hospital, Abuja.

3 Department of Medical Microbiology and Parasitology, Federal Medical Centre, Abuja.

 

 

ARTICLE INFO

ABSTRACT

 

Article No.: 110521113

Type: Research

Full Text: HTML;EPUB

 

Background: Production of extended-spectrum β-lactamases (ESBLs) is the most common mechanism of resistance to third-generation cephalosporins among Enterobacteriaceae including Klebsiella pneumonia and this presents therapeutic challenges managing infections caused by these strains of bacteria.

Aim: To determine the prevalence, antibiotic susceptibility profile and major ESBL encoding genes among Klebsiella pneumoniae in clinical specimens.

Methods: Four hundred (400) consecutive and non-duplicate isolates of Klebsiella pneumoniae from clinical specimens were identified by standard laboratory methods at the National Hospital Abuja, subjected to antimicrobial susceptibility testing using the Kirby-Bauer disc diffusion method and identified ESBL phenotypes were confirmed using E-test. Multiplex PCR was used to detect ESBL genes.

Results: Out of the 400 Klebsiella pneumoniae isolates, 114 (28.5%) were ESBL producers, out of which 111 (97.4%)  were sensitive to meropenem, 101 (88.6%) to amikacin, 100 (87.7%) to fosfomycin, 96 (84.2%) to tigecycline, and 58 (50.9%) to nitofurantoin.  All the ESBL producers were resistant to cefotaxime while 107 (93.9%)  and 105 (92.1%) were resistant to amoxicillin-clavulanate, and ceftazidime respectively . There was a significantly higher distribution of multidrug resistance among ESBL producing isolates compared to non-ESBL producing isolates (chi-square =63.29, p-value = 0.0001). The distribution showed that 78 (70.3%) had the blaSHV gene, 99 (89.2%) had the blaCTX-M gene, 88 (79.3%) had the blaTEM gene and 3 (2.6%) had none of the major bla genes.

Conclusion: This study showed a relatively high prevalence of ESBL-producing Klebsiella pneumoniae isolates and a significant occurrence of multidrug-resistant Klebsiella pnuemoniae. Meropenem and amikacin are excellent therapeutic choices for empirical therapy of ESBL-producing Klebsiella pneumoniae infections and their use should be properly guarded through efficient infection control and antimicrobial stewardship..

 

Accepted:  05/11/2021

Published: 15/11/2021

 

*Corresponding Author

Enyinnaya, SO

E-mail: stellaoikedichi@gmail.com

 

Keywords:

Klebsiella pnuemoniae; ESBL; Multiplex-PCR; Antibiotic Resistance.

 

 

 

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Cite this Article: Enyinnaya SO; Iregbu KC; Uwaezuoke, NS; Abdullahi, N; Lawson, SD (2021). Molecular Detection and Antibiotic Susceptibility Profile of ESBL-producing Klebsiella pneumoniae Isolates in a Central Nigerian Tertiary Hospital. Greener Journal of Medical Sciences, 11(2): 181-186.

 

 


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