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Ebede et al

By Ebede, SO; Emenuga, VN; Nwobodo, HA; Imanyikwa OEI; Offor, CC; Usige, E; Oguwike, RC; Oguwike, FN; Okeke, JC (2022).

Greener Journal of Medical Sciences

Vol. 12(1), pp. 1-5, 2022

ISSN: 2276-7797

Copyright ©2022, the copyright of this article is retained by the author(s)

https://gjournals.org/GJMS

 

 

 

 

 

Evaluation of Bacterial Contaminants of over Riped Pear (Dacryodes edulis) and Their Effect in the Gastrointestinal System of Children.

 

 

Ebede S.O1, Emenuga V. N2, Nwobodo H.A3, Imanyikwa O.E.I3, Offor C.C4, Usige E5, Oguwike R.C6, Oguwike F.N7, Okeke J.C7.

 

 

1.      Dept. of Medical Microbiology, University Teaching Hospital, Ituku Ozalla Enugu, Enugu State.

2.      Dept. of Medical Laboratory Sciences, Faculty of Health Science and Technology, College of Medicine University of Nigeria Enugu Campus, Nigeria.

3.      Dept. of Medical Microbiology, Faculty of Health Science and Technology, College of Medicine. Enugu State University Teaching Hospital Enugu, Nigeria.

4.      Dept. of Medical Biochemistry, Faculty of Basic Medical Science. Chukwuemeka Odumegwu, Ojukwu University Uli Campus, Anambra State.

5.      Dept. of Anatomy, Faculty of Basic Medical Sciences. Chukwuemeka Odumegwu, Ojukwu University Uli Campus, Anambra State.

6.      Dept. of Pharmacology, Faculty of Pharmaceutical Sciences. Chukwuemeka Odumegwu, Ojukwu University Igbariam Campus, Anambra State.

7.      Dept. of Physiology, Faculty of Basic Medical Sciences. Chukwuemeka Odumegwu, Ojukwu University Uli Campus, Anambra State.

 

 

ARTICLE INFO

ABSTRACT

 

Article No.: 011922006

Type: Research

Full Text: PDF, HTML, EPUB, PHP

Dacryodes edulis exists as a fruit tree native to Africa, sometimes called Ube. (Igbo, Nigeria) or African bush pear.

 The occasional outbreak of diarrhea as a result of gastrointestinal infection among children of age 8 months to 2 years necessitated this study. Evaluation of bacterial contaminants in over riped pear (Dacryodes edulis) and their effect in the gastrointestinal system of children was investigated using thirty (30) children within the age range of 8 months to 2 years. The investigation was carried out using swab sticks to collect samples from over riped pears (50 samples) and also isolation of bacteria and fungi from children who had loose or watery stool after ingestion of over riped pears.

The samples from over riped Dacryodes edulis and infected children were cultured in Sabouraud agar, Nutrient agar and MacConkey agar. They were left to incubate for 24-48 hours. Many methods were adopted in identification of various bacteria and micro flora contaminants with their colonies counted.

   Results indicated that yeast organisms predominated, while other organisms   such as Staph. epidermidis, aerobic mesophiles and Staphylococcus mirabilis were also present. It could be deduced that these bacterial and fungi contaminants could be responsible for watery stool and gastrointestinal disorder in some of the children.

 

  

 

Accepted:  22/01/2022

Published: 17/02/2022

 

*Corresponding Author

Dr. V. N Emenuga

E-mail: veronica.emenuga@ unn.edu.ng

Phone: 08035506341

 

Keywords:

Dacryodes edulis, Fungi, Agar, Bacteria, Children.

 

 

 

 

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REFERENCES

 

1.      Aguoru C.E, S. Maaji and J.O Olasan (2015): Bacteria contaminants on surfaces of some edible fruits sold in Markurdi metropolis, Benue state Nigeria. International Journal of Current Microbiology and Applied Sciences. ISSN: 2319-7706. Vol. 4 Number 6 pp 334-340.

 

2.      Baker F.J, Silverton R.E (1998): Method of anaerobic cultivation of Bacteria. Introduction to Medical Laboratory Technology 7th Edition. Butter Worth publication. P 290-291.

 

3.      Catherine Texier, Frédéric Delbac (2010), Current Opinion in Microbiology, Elsevier:3(9-12) .

 

4.      Crewe W (1977). Examination of the faeces for protozoa. A Guide to Human Parasitology for Medical Practitioners 10th edition. Published in Great Britain for H. K. Lewis ISBN 0718604326 pg. 177-179.

 

5.      Cruishank R, (1973): Microbiology and Medicine. Medical Microbiology. Churchill living stone, 23 Rowelston Edinburgh Terrance. Vol. 1 p4, 10, 110, 134-136.

 

6.      National Research Council (NRC, 2008). “Butter fruit”. Lost crops of Africa Volume III. Fruits, lost crops of Africa 3 National academics. Press. doi: 10, 17226/11879, ISBN 978-0-309-10596-5.

 

7.      Oguwike F.N, Nwobodo H.A, Emenuga V.N, Offor C.C, Ifedi I.C (2018). Comparative study of the micro flora of the oral cavity in sick and healthy subjects in Enugu state. Greener Journal of Medical Sciences. Vol. 8 no 4 p057-060.


 

Cite this Article: Ebede, SO; Emenuga, VN; Nwobodo, HA; Imanyikwa OEI; Offor, CC; Usige, E; Oguwike, RC; Oguwike, FN; Okeke, JC (2022). Evaluation of Bacterial Contaminants of over Riped Pear (Dacryodes edulis) and Their Effect in the Gastrointestinal System of Children. Greener Journal of Medical Sciences, 12(1): 1-5.

 


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