Greener Journal of Agricultural Sciences Vol. 6 (10), pp. 304-311, November 2016.
Manuscript Number: 101916186
Aflatoxin and Fumonisin Contamination of at-harvest and Storage Beans in Babati District, Northern Tanzania
Department of Science and Business Management, Mbeya University of Science and Technology, P.O. Box 131, Mbeya, Tanzania.
The natural occurrence of total aflatoxin and fumonisin was determined in 38 bean at-harvest samples and 106 storage beans samples in the year 2013 in Babati District Northern Tanzania. Quantification for total aflatoxin and fumonisin was done using Enzymes Linked Immunosorbent Assay - ELISA (Reveal AccuScan® Neogen, USA), and the results were confirmed using Liquid Chromatography Tandem Mass Spectrometer (LC-MS/MS). Eighteen percent (7/38) of bean at harvest samples were contaminated with up to 3 µg/kg aflatoxins and no bean sample was contaminated with fumonisins. There was a significant correlation (p < 0.05) between the concentration of aflatoxins and fumonisins with climatic zones and agronomic practices. Only bean samples from the high altitude high rain zone were contaminated with aflatoxins (mean level of 1.53 µg/kg). For storage beans, samples from Seloto village were associated with higher aflatoxin concentration (mean of 3.74 µg/kg) and those from Long village were associated with higher fumonisin levels (mean of 9.0 mg/kg). These results indicate that beans consumers in the study area are exposed to the danger of chronic exposure to aflatoxin and fumonisin poisoning. Thus, those practices that reduce contamination should be adopted by all farmers in the study area to reduce the health hazards associated with consumption of contaminated beans. This also calls for further research to investigate human exposure to aflatoxin and fumonisin in the study area.
Keywords: Aflatoxin, fumonisin, at-harvest, storage practices, beans, Babati, Tanzania.
Abbas HK, Shier WT, Cartwright, RD (2007). Effect of temperature, rainfall and planting date on aflatoxin and fumonisin contamination in commercial Bt and non-Bt corn hybrids in Arkansas. Phytoprotection 88 (2): 41-50
Aiat N (2006). Genetic Variability among Three Species of Aspergillus. Seed born Aspergillus Sp. and Aflatoxins Associated with Dry Beans (Phaseolus vulgaris L.). J. Appl. Sci. Res. 2(11): 874-878.
Atanda O, Makun HA, Ogara IM, Edema M, Idahor KO, Eshiett ME and BF Oluwabamiwo (2013). Fungal and Mycotoxin Contamination of Nigerian Foods and Feeds. In: Makun H A (Ed). Mycotoxin and Food Safety in Developing countries. InTech Janeza Trdine 9, 51000 Rijeka, Croatia. 1: 15-50.
Atukwase, A, Kaaya, AN and Muyanja, C (2009). Factors associated with fumonisin contamination of maize in Uganda. J. Sci. Food Agr. 89: 2393–2398.
Bruns HA (2003). Controlling aflatoxin and fumonisin in maize by crop management. J. Toxicol. Toxin. Rev. 22(2-3): 153 -173.
East African Community Final Draft East African Standards (EAC) FDEAS No. 46/2011 of 2011 specifying requirements and methods of sampling and test for dry common beans (Phaseolus vulgaris Linn) intended for human consumption. [file:///C:/Users/user/Downloads/Dry_beans_Specification.pdf]. 2011b; site visited on 04-08-2014.
Egal, S, Hounsa A, Gong YY, Turner PC, Wild CP, Hall AJ, Hell K and Cardwell K (2005). Dietary exposure to aflatoxin from maize and groundnut in young children from Benin and Togo, West Africa. Int J Food Microbiol. 104: 215-224.
European Commission Commission Regulation (EC) No. 165/2010 of November 2010 setting maximum levels for certain contaminants in food stuffs as regards aflatoxins. Official Journal of the European Union, I. 2010; 50:8-12 [http://ec.europa.eu/food/food/chemicalsafety/contaminants/guidance-2010.pdf] site visited on 28/05/2015.
Fandohan P, Ahouansou R, Houssou P, Hell K, Marasas WFO and Wingfield MJ (2005). Impact of mechanical shelling and dehulling on Fusarium infection and fumonisin contamination in maize. Food Addit Contam. 23: 451-421.
FAO. FAOSTAT database: [http://faostat3.fao.org/faostat-gateway/go/to/download/Q/QC/E]. 2013; site visited on 04–08-2015.
Gil-Sotres F, Trasal-Cepeda C, Leiros MC and S Seoane (2005). Different approaches to evaluating soil quality using biochemical properties. Soil Biol Biochem. 2005; 37: 877- 892.
Hell K and Mutegi C (2011). Aflatoxin control and prevention strategies in key crops of Sub-Saharan Africa. Afr. J. Microbiol. Res. 5(5): 459-466.
Hell K, Cardwell KF, Setamou M and Poehling HM (2000). The influence of storage practices on aflatoxin contamination in maize in four climatic zones of Benin West Africa. J Stored Prod Res. 36:365-382.
Hell K, Ognakossan KE, Tonou AK, Lamboni Y, Adabe, KE and Coulibaly O (2010). Maize Stored Pests Control by PICS-Bags: Technological and Economic Evaluation. Presentation at the 5th World Cowpea Conference, Saly, Senegal, 27 September–1October 2010. [https:// ag.purdue.edu/ipia/ pics/documents/ presentation-%20hell%20-wcc%202010-%2029%20sept.pdf] site visited on 16/05/2016.
International Agency for Research on Cancer (IARC) (1993). Ochratoxin A. Monographs on the Evaluation of Carcinogenic Risks to Humans, Some Naturally Occurring Substances. Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins. 56: 489–521.
Janusauskaite D, Kadziene G and O Auskalniene (2013). The effect of tillage system on soil Microbiota in relation to soil structure. Pol J Environ Stud. 22(5): 1387-1391.
Kaaya AN, Kyamuhangire W and Kyamanywa S (2006). Factors affecting aflatoxin contamination of harvested maize in the three climatic zones of Uganda. J Appl Sci. 11: 2401–2407.
Kaaya AN and Kyamuhangire W (2006). The effect of storage time and climatic zone on mould incidence and aflatoxin contamination of maize from traders in Uganda. Int J Food Microbiol. 110:217-223.
Kimanya ME, De Meulenaer B, Roberfroid D, Lachat C and P Kolsteren (2010). Fumonisin exposure through maize in complementary foods is inversely associated with linear growth of infants in Tanzania. Mol Nutr Food Res. 54: 1659–1667.
Marasas WFO, Gelderblom WCA, Shephard GS and HF Vismer (2008). Mycotoxins: A Global Problem. In: Leslie J F, Bandyopadhyay R and A Visconti (Eds). Mycotoxins: Detection methods, Management, Public Health and Agricultural Trade. Cromwell press, Townbridge, UK. 4: 29-39.
Milani JM (2013). Ecological conditions affecting mycotoxin production in cereals: a review. Vet. Med-Czech. 58(8): 405–411.
Nyangi C, Beed F, Mugula JK, Boni S, Koyano E, Mahuku G, Sulyok M and B
Mateete Assessment of Pre-harvest Aflatoxin and Fumonisin Contamination of
Maize in Babati District, Tanzania. Afr. J. Food Agric. Nutr. Dev. 2016; 16(3):
Nyinawabali F A survey of fungi and mycotoxins in selected food commodities from Rwanda. Published Dissertation for award of MSc Degree at University of Johannesburg, South Africa.
Omar HEM (2013). Mycotoxins-Induced Oxidative Stress and Disease. In: Mycotoxin and Food Safety in Developing countries (Edited by Makun, H.A.) InTech Janeza Trdine 9, 51000 Rijeka, Croatia. pp. 75-104.
Orsi RB, Corrêa B, Possi CR, Schammass EA, Nogueira JR, Dias SMC and Malozzi MAB (2000). Mycroflora and occurrence of fumonisins in freshly harvested and stored hybrid maize. J Stored Prod Res. 36: 75-87.
Stössel P (1986). Aflatoxin contamination in soybeans: role of proteinase inhibitors, zinc availability, and seed coat integrity. Appl. Environ. Microbiol. 52: 68–72.
Tseng TC, Tu JC and Tzean SS (1995). Mycoflora and mycotoxins in dry bean (Phaseolus vulgaris) produced in Taiwan and in Ontario, Canada. Bot Bull Acad Sinica. 36:229–234.
Udoh JM, Cardwell KF and Ikotun T (2000). Storage structures and aflatoxin content of maize in five climatic zones of Nigeria. J Stored Prod Res. 36(2): 187-201.
Ueno Y, Iijima K, Wang SD, Sugiura Y, Sekijima M and T Tanaka (1997). Fumonisins as a possible contributory risk factor for primary liver cancer: A 3-year study of corn harvest in Haimen, China, by HPLC and ELISA. Food Chem Toxicol. 35: 1143 – 1150.
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