Greener Journal of Agricultural Sciences

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Beksisa et al

Greener Journal of Agricultural Sciences

Vol. 9(3), pp. 302-308, 2019

ISSN: 2276-7770

Copyright ©2019, the copyright of this article is retained by the author(s)

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Farm based outbreak study and Genetic characterization of O serotype Foot and mouth diseases virus isolated from clinical cases in dairy farms of Welmera district, Central Oromia, Ethiopia



Beksisa Urge, Fufa Dawo, Daniel Gizaw, Melaku Sombo, Ayelech Muluneh , Abdi Aliyi, Bayata Senbeta, Tsion Bilata


Ethiopian Institute of Agricultural Research, Holeta Agricultural Research Center






Article No.: 072319141

Type: Research

DOI: 10.15580/GJAS.2019.3.072319141



Foot and mouth disease (FMD) is a trans-boundary viral disease that causes huge economic losses.  A field based outbreak study was conducted in September 2016/ 2017 to identify and genetically characterize O serotype from clinical and asymptomatic cases at Welmera district Menagesha kolobo kelebe of central Oromia. The investigation was purposively conducted in the respective farms where outbreak occurred. Accordingly, 50 dairy cattle were clinically examined and about 20 (40%) animals showed sign and clinical lesions of the disease.   Accordingly, active epithelial tissues and probang samples were taken from acutely and severely infected animals for virus isolation, typing and genetic detection. These clinical samples were processed for antigen typing and O serotype was isolated from clinical and asymptomatic cases in the farm.  The genetic material of this strain was tested by   rRT- PCR and the viral genome was detected in the epithelial tissues and probang samples.  In conclusion, the study showed that O serotype was circulating in the district. Therefore, foot and mouth disease control should be established based on the availability of potent and matched vaccines against the circulating field strains.


Submitted: 23/07/2019

Accepted:  03/08/2019

Published: 26/08/2019


*Corresponding Author

Beksisa Urge

E-mail: beksisaurge@



Cattle; FMDV; Welmera; Serotypes; Outbreak



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Cite this Article: Beksisa, U; Fufa, D; Daniel, G, Melaku, S; Ayelech, M; Abdi, A; Bayata, S; Tsion, Bilata (2019). Farm based outbreak study and Genetic characterization of O serotype Foot and mouth diseases virus isolated from clinical cases in dairy farms of Welmera district, Central Oromia, Ethiopia. Greener Journal of Agricultural Sciences 9(3): 302-308,


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