Greener Journal of Agricultural Sciences

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Chukunda and Onyeizu

Greener Journal of Agricultural Sciences

Vol. 9(1), pp. 113-118, 2019

ISSN: 2276-7770

Copyright ©2019, the copyright of this article is retained by the author(s)

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Influence of culture media, temperature and light/darkness on the mycelial growth of Lasiodiplodia theobromae (Pat.)


1Chukunda, F.A.; 2Onyeizu, U.R.


1Department of Forestry and Environment, Faculty of Agriculture, Rivers State University of Science and Technology, Port Harcourt, Nigeria.

2Department of Environmental Management & Toxicology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria





Article No.: 031419046

Type: Research

DOI: 10.15580/GJAS.2019.1.031419046



In this study, the effects of culture media, temperature and light and darkness on mycelial growth of L. theobromae were evaluated. Results of potato dextrose agar (PDA) and potato dextrose agar stem exudates (PDASE) were found suitable for mycelial growth of the L. theobromae. The fungus grew from 20-40oC with optimum growth observed at 25-35oC on both media. On the 10th day, mycelial growth at 25-35oC was (15.6mm ± 0.02 - 30.6mm ± 0.05; 18.4mm ± 0.28 - 32.5mm ± 0.10) while mycelial growth recorded with PDASE was (18.2mm ± 0.23 – 31.0mm ± 0.25; 22.8mm ± 0.02 – 38.2mm ± 0.40). There were no significant effects of light and darkness on the mycelial difference (P≤ 0.05) on the growth of L. theobromae. It is recommended from this research work that amended potato dextrose agar (PDA) with stem exudates promoted the growth of test fungus L. theobromae and hence should be used as culture medium for fungi at 35oC.


Submitted: 14/03/2019

Accepted:  21/03/2019

Published: 30/03/2019


*Corresponding Author

Chukunda, F.A.

E-mail: onyifrank2002@

Phone: 08037501179


Keywords: Culture media, Potato dextrose agar stem exudates (PDASE), potato dextrose agar (PDA)




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Cite this Article: Chukunda, FA; Onyeizu, UR (2019). Influence of culture media, temperature and light/darkness on the mycelial growth of Lasiodiplodia theobromae (Pat.). Greener Journal of Agricultural Sciences 9(1): 113-118,

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