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Makanju et al

Greener Journal of Agricultural Sciences

Vol. 11(2), pp. 108-113, 2021

ISSN: 2276-7770

Copyright ©2021, the copyright of this article is retained by the author(s)







Haematology and Serum biochemistry of broilers fed diets containing cassava peels and cassava leaf meal



1Makanju T.D.; 2Abu O.A.; 3Olaleru, I.F.; 1Amusa, H.O.



1Oyo State College of Agriculture and Technology, Igboora, Oyo state.

2Department of Animal Science, University of Ibadan, Oyo State, Nigeria

3Faming Systems Research Program, National Root Crops Research Institute, Umudike, Abia State. Nigeria






Article No.: 071221065

Type: Research



A 7 weeks feeding trial was conducted in a completely randomized design to evaluate the haematological parameters and serum biochemistry parameters of 7-day old Abor-acre broiler chicks fed with different diets containing cassava peels and cassava leaf meal. The birds were assigned to four dietary treatments with three replicates and 15 birds per replicate. The birds in the control treatment (Treatment 1) were fed diets that did not contain cassava peel or cassava leaf meal, birds in treatment 2 were fed with diets containing cassava peel to replace maize by 20% and cassava leaf meal to replace soya bean meal by 20%, birds in treatment 3 were fed diets containings cassava leaves to replace soya bean meal by 20% and birds in treatment 4 were fed diets containing cassava peel to replace maize by 20%. The chicks were mass brooded at day old and fed with commercial broiler starter feed for one week before being randomly allotted to various dietary treatments. The birds were fed the experimental starter diet for 3 weeks and the experimental finisher diets for 4 weeks.

With the exception of lymphocytes and neutrophils, there was no significant differences (p>0.05) in the haematological parameters across the dietary treatments. All the serum biochemistry parameters were not significantly different (p>0.05) across the dietary treatments.


Accepted:  15/07/2021

Published: 31/07/2021


*Corresponding Author

Tomilola Makanju

E-mail: okeoluwatomilola@; okeoluwatomilola@

Phone: +2348066639150



cassava peels; cassava leaves; broiler; haematology; serum biochemistry






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A.O. A.C. 2000. Official Method of Analysis 16th ed. Association of Official Chemists. Washington D. C.


Aderemi, F. and Alabi, O. 2020. Effects of graded levels of cassava peels fortified with moringa as a replacement to wheat bran on performance, digestibility and blood profile of broilers. Journal of Agricultural Science and Technology 3: 210.215 (2013)


Adeyemo, A. A. and Sani, A. 2013 hematological parameters and serum biochemical indices of broiler chickens fed Aspergillus niger hydrolyzed cassava peel meal based diets. IJRRAS 15 (3)


Akinfala, E. O. and Tewe, O. O. 2004. Supplemental effects of feed additives on the utilization of whole cassava plant by growing pigs in the tropics. Livestock Research for Rural Development 16 (10) 2004


Akinfala, E. O., Matanmi, O. and Tinuala, J. A. 2011. Nutrient characterization of cassava plant meal and its utilization by broiler chickens in the tropics. Livestock Research for Rural Development 23 (11)2011.


Ameen, S. A., Adedeji, A. A., Akingbade, A. A., Olayemi, T. B., Ojedapo, L.O. and Aderinola, O. A. 2007. The effect of different feeding regimes on hematological parameters and immune status of commercial broilers in the derived savannah zone of Nigeria. Proceeding of 32nd Annual Conference of The Nigerian Society for Animal Production. March 18-21, University of Calabar, Nigeria, pp: 176-178


Anaeto, M. and Adighibe, L. C. 2011. Cassava root meal as substitute for maize in layers ration. Rev. Bras. Cienc. Avic. vol.13 no.2.


Bhuiyan, M. M. and Iji, P. A. 2015. Energy Value of Cassava Products in Broiler Chicken Diets with or without Enzyme Supplementation. Asian-Australas J Anim Sci. 2015 Sep; 28(9): 1317–1326. doi: 10.5713/ajas.14.0915


CGIAR. Cassava. Available from


Cooper, G. R. and McDaniel, V. 1970. Standard methods in serum enzyme activity. Clinical Chemistry Vol. 6 pg. 159


Egbunike, G. N., Agiang, E. A., Owosibo, A. O. and Fatufe, A. A. 2009. Effect of protein on performance and hematology of broilers fed cassava peel based diets. Arch. Zootec. 58 (224): 655-662


Emenalum, O. O. Esonu, B. O., Etuk, E. B. and Anaba, C. 2009. Effect of Mucuna prurience (velvet beans) leaf meal on performance and blood composition of finisher broiler chickens. Nigerian Journal of Animal Production. 36 (1): 52-60


Esonu, B. O., Emenalom, O. O., Udedibe, A. B. I., Herbert, U., Ekpor, C. F., Okoli, I. C. and Iheukwumere, F. C. 2001. Performance and blood chemistry of weaner pigs fed raw mucuna beans (velvet beans) meal. Trop. Anim. Prod. Invest. 4:49-54


FAO (2018) Food Outlook - Biannual Report on Global Food Markets – November 2018. Rome, p. 104 License: CC BY-NC-SA 3.0 IGO.


Food and Agriculture Organization of the United Nations (FAO). FAOSTAT Statistical Database, Statistical Division.Rome (2019).


Harper, H. A. 1982. Review of physiological chemistry, 15th Edn. Lange Medical Publications pp. 553


Jain, N. C. 1986. Schalm’s Veterinary Haematology. 4th Edn, Lea and Febiger, Philadelphia.


Kelly, R. W. 1979. Veterinary Clinical diagnosis, 2nd Edition, Tindal, London, pp. 266-267.


McDonald, S. 1996. Complete blood count, Avian Quarterly.


Mitruka, B. M. and Rawnsley, H. M. 1977. Clinical, Biochemical and Hematological reference values in normal experimental animals. Masson Publishing U. S. A. Inc. New York.


Nowaczewski, S. and Kontecka, H. (2012). Hematological indices, size of erythrocytes and haemoglobin saturation in broiler chickens kept in commercial conditions. Animal Science Papers and Reports Vol. 30 (2012), no. 2:181-190. Institute of Genetics and Animal Breeding, Jastrzebiec, Poland.


Odunsi, A. A. 2003. Blend of bovine blood and rumen digesta as a replacement for fishmeal and groundnut cake in layer diets. International Journal of Poultry Science, 2 (1): 58-61


Ogbuewu, I. P., Uchegbu., M. C., Ezuma, C. C and Opara, M. N. 2008. Physiological responses of finisher broilers to yam peel meal: Hematology and serum biochemistry. Electronic Journal of Enviromental, Agriculture and Food Chemistry. ISSN: 1579-1664


Okorie, K. C., Nkwocha, G. A. and Ndubuisi, E. C. 2011. Implications of feeding different dietary levels of cassava leaf meal on finisher broilers: performance, carcass, hematology and serological profiles. Global Research Journal of Science 1: 58-66, 2011


Rajurker, S., Relche, D. S., Maini, S. and Ravikanth, K. 2009. Acute toxicity studies of polyherbal formulation (Methioreb premix). Vet. World 2:58.59


Reinhold, J. G. 1953. Standard methods of clinical chemistry, Reiner, M. ed. Volume 188, Academic Press.


Rej, R. and Holder, M. 1983. Aspartate amino transferase. In Methods of enzymatic analysis. 3rd ed. H. U. Bergmeyer and M. Grass (Eds.). Weinheim, Velacheime, 3:416-433


SAS Institute. 2000. SAS/STAT Guide for personal computers, Version and Edition Cary. North Carolina SAS Institute.


Schalm, O.W., Jain N.C., Carol E.J. Veterinary Haematology. 3rd Edition. Publisher, 1975, Lea and Febiger Philadelphia.


Silva, P. R. L., Freitas, O. C., Laurentiz, A. C., Junquiera, O. M. and Fagliari, J. J. 2009. Blood serum components and serum protein tests of Hybro-PG broilers of different ages. Brazilian Journal of Poultry Science. Vol. 9, No. 4, pp: 229-232


Tewe, O. O. and Egbunike, G. N. 1992. Utilization of cassava in non-ruminant livestock feeds. In proceedings of cassava as livestock feed in Africa. Workshop of IITA/IICA/University of Ibadan on the potential of cassava as livestock feed in Africa (Eds.) Hahn, S. K., Reynolds, L. and Egbunike, G. N. pp. 28-38.




Cite this Article: Makanju TD; Olaleru, IF; Amusa, HO (2021). Haematology and Serum biochemistry of broilers fed diets containing cassava peels and cassava leaf meal. Greener Journal of Agricultural Sciences 11(2): 108-113.



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