Greener Journal of Agricultural Sciences

Excellence and Timeliness


Change Language

Meleta and Abera

Greener Journal of Agricultural Sciences

Vol. 9(4), pp. 436-446, 2019

ISSN: 2276-7770

Copyright ©2019, the copyright of this article is retained by the author(s)

DOI Link:







Effects of Rhizobium Inoculation and Phosphorus Fertilizer rates on Nitrogen Fixation and Nutrient up take of Chickpea (Cicer arietinum L.) at Goro, Bale Zone, Oromia Regional State.



Tamiru Meleta 1*; Girma Abera 2


 1 Sinana Agricultural Research Centre, Bale-Robe, Ethiopia

2 Hawassa University College of Agriculture, Hawassa






Article No.: 101419186

Type: Research

DOI: 10.15580/GJAS.2019.4.101419186



Chickpea response to two varieties of chickpea (Arerti and Habr), two Rhizobial inoculants (EAL 018 and EAL 029) and five P rate (0, 15, 30, 45 and 60 kg P2O5 ha-1) were studied in split plot arrangements. Main plot consisted of varieties whereas P2O5 levels and Rhizobium strains were kept in subplot. The experiment was laid out at Goro, Bale Zone, Oromia Regional State  during 2015/16  with the objective to determine the effectiveness of Rhizobium strains and phosphorus fertilizer application on two varieties of chickpea (Arerti and Habru).The nodulation rating, nodule volume and color were not significantly affected due to varieties. However, significant variation (P <0.05) was observed in number of nodule per plant (NNPP) and nodule dry weight (NDW) between varieties of chick pea, maximum NNPP and NDW was obtained from Arerti variety. All the nodulation parameters were significantly (P<0.05) affected due to rhizobium inoculation and P2O5 except the nodule color. Maximum nodulation parameters were recorded at 45 and 60 kg P2O5 ha-1and inoculation by EAL 029 strain except the nodule color. Significant interaction effect was observed between variety and Rhizobium inoculation for nodule rate (NR), NDW between variety and P2O5, rhizobium and P2O5 and also interaction effect between varieties, Rhizobium and P2O5 on NR and NDW. The N and P uptake at mid flowering, %Ndfa, grain, straw and total N and P content and crude protein content were significantly affected by variety, rhizobium inoculation and application of P2O5. Maximum response was obtained from EAL 029 strain inoculations and application of 45 kg P2O5 ha-1 for N uptake, seed N and P uptake were maximum at 45and 60 kg P2O5 ha-1 respectively. The current investigation indicated that Arerti variety inoculation with Rhizobium strain EAL 029 along with application of P2O5 at rate of 45 kg ha-1 found to be appropriate for chickpea production in the study area. The current investigation indicated that the use of P2O5 with Rhizobium inoculation as a nutrient management strategy could increase chick pea production. Since the experiment was conducted only for one year, we suggest the results to be verified with more varieties of chickpea and rhizobium strain under the same agro-climatic conditions.


Submitted: 14/10/2019

Accepted:  29/10/2019

Published: 01/11/2019


*Corresponding Author

Tamiru Meleta

E-mail: tamish09@ gmail. com


Keywords: Phosphorus; Rhizobia strain; Nutrient uptake; Chickpea; Bale Robe.



Return to Content       View [Full Article - PDF]  

[Full Article - HTML]               [Full Article - EPUB]

Post-Publication Peer-review Rundown

View/get involved, click [Peer-review]




Alemu Desa. 2009. Effect of phosphorus application and rhizobium inoculation on nodulation, yield and yield related traits of fenugreek (Trigonella foenum-graecum L.) in sinana, south eastern Ethiopia. Thesis, Haramaya University, Haramaya, Ethiopia.


Ali M.E., Khanam D. M., Bhuiya H. A., Khatun M.R. and Talukder M.R. 2008. Effect of Rhizobium inoculation on different varieties of garden pea (Pisum sativum L.). J. Soi. Nature2:30-33.


Azma F. 2006. Added nitrogen, it is occurrence mechanism and implications to fate of N in the soil plant system .Pak.J.Agron.1:54-59.


Basher K., Ali S., USAir A. 2011. Effect of different phosphorus levels on xylem sap components and their correlation with growth variables of mash bean. Sarhad Journal of Agriculture, Vol. 27, and No. 4.


Bulter T.J. and Evers G. 2004. Inoculation, nodulation, nitrogen fixation and transfer. Texas cooperative extension (press).


CSA (Central Statistics Authority). 2015. Agricultural Sample Survey. Reports on area under cultivation, yield and production of major crops for main (Maher) season, Ethiopia.


Haruna I. M. and Aliyu L. 2011. Yield and economic returns of sesame (Sesamumindicum L.) as influenced by poultry manure, nitrogen and phosphorus at Samaru,  Nigeria. Elixir Agric., 39: 4884-4887.


Havlin J.L., Beaton J.D., Tisdale S.L. and Nelson W.L. 1999. Soil Fertility and Fertilizers: An Introduction to Nutrient Management, Prentice Hall, New Jersey. pp 499.


Hussain M.D., Roman M.M. & Fujita M. 2007. Comparative investigation of glutathione S-transferees, glyoxalase and alliance activities in different vegetable crops. Journal of Crop Science and Biotechnology. 10, 21-28, ISSN 2005-8276.


Jennings J. 2004. Forage legume inoculation. In: Agriculture and Natural Resources University of Arkansa press. UK.


Kenani G., Berkeley E., Imia M. 2012. Genetic diversity and population structure of Ethiopian chickpea (Cicero arietinum L.) germplasm accessions from different geographical origins as revealed by microsatellite markers. Plant Mol. Biol. Rep. 30: 654 – 665.


Qin L., Jiang H., Tian J., Zhaod J. and Liao H. 2011. Rhizobia enhance acquisition of phosphorus from different sources by soybean plants. Plant Soil 349:25–36.


Redden R.J. and Berger J.D. 2007. History and origin of chick pea .In:Yadaw et al.,(eds) chick pea breeding and management pp 1.13.CABI Walling ford, UK.


Sarawg S.K., Tiwari P.K. and Ttripathi R.S. 1999. Uptake and balance sheet of nitrogen and phosphorus in gram as influenced by phosphorus, biofertilizers and micronutrients under rain fed condition. Indian J. Agron., 44(4): 768-772.


SAS Inst.Inc. 2002. SAS online doc. 9. Cary, In. NC: SAS Institute Inc. USA.


Singh A., Baoule A., Ahmed HG., Dikko AU., Aliyu U.,Sokoto MB., Hassan J., Musa M., Haliru B. 2011. Influence of phosphorus on the performance of cowpea (Vigna unguiculata L. Walp.) varieties in the Sudan savanna of Nigeria. Agri. Science. 2(3): 313-317.


Tahir M., Javed M. R., Tanver A., Nadem M. A. andWasaya A., Bukhari S.A.H. and Rehman J. U. 2009. Efect of different herbicides on weeds, growth and yield of spring planted maize (Zea mays L.). Pak. J. Life Soc. Sci. 7(2): 168-174.


Cite this Article: Meleta, T; Abera, G (2019). Effects of Rhizobium Inoculation and Phosphorus Fertilizer rates on Nitrogen Fixation and Nutrient up take of Chickpea (Cicer arietinum L.) at Goro, Bale Zone, Oromia Regional State. Greener Journal of Agricultural Sciences 9(4): 436-446, 




Call for Articles/Books

Call for Scholarly Articles

Authors from around the world are invited to send scholary articles that suits the scope of this journal. The journal is currently open to submissions and will process and publish articles daily, immediately they are ready.

The journal is centered on quality and goes about its processes in a very timely fashion. Seasoned editors/reviewers will be consulted to review each article(s), profer quality evaluations and polish the articles with expertise before publication.

Use our quick submit button to submit or simply send your article(s) as an e-mail attachment to or

Call for Books

You are also invited to submit your books for online or print publication. We publish books related to all academic subject areas.    Submit as an e-mail attachment to


Login Form

Other Journals